Starvation results in decreased initiation factor activity in rat skeletal muscle

D. Eugene Rannels; Anthony E. Pegg; Stephen R. Rannels

doi:10.1016/S0006-291X(76)80181-7

Starvation results in decreased initiation factor activity in rat skeletal muscle

D. Eugene Rannels, Anthony E. Pegg, Stephen R. Rannels

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Activity of a factor forming a nitrocellulose filter-bound complex with [³⁵S]met-tRNA^met_f and GTP was estimated in the post-ribosomal supernatant of psoas and heart muscle. Complex formation required initiator tRNA and GTP or GMP-P(CH₂)P; it was inhibited by Mg²⁺, GDP, aurintricarboxylic acid, spermine and spermidine. Starvation reduced complex-forming activity of supernatants from psoas but not heart muscle. Reduced activity was not due to increased dilution or deacylation of met-tRNA^met_f or to increased hydrolysis of GTP by extracts from starved animals.

Original language	English (US)
Pages (from-to)	1481-1489
Number of pages	9
Journal	Biochemical and Biophysical Research Communications
Volume	72
Issue number	4
DOIs	https://doi.org/10.1016/S0006-291X(76)80181-7
State	Published - Oct 18 1976

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/S0006-291X(76)80181-7

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title = "Starvation results in decreased initiation factor activity in rat skeletal muscle",

abstract = "Activity of a factor forming a nitrocellulose filter-bound complex with [35S]met-tRNAmetf and GTP was estimated in the post-ribosomal supernatant of psoas and heart muscle. Complex formation required initiator tRNA and GTP or GMP-P(CH2)P; it was inhibited by Mg2+, GDP, aurintricarboxylic acid, spermine and spermidine. Starvation reduced complex-forming activity of supernatants from psoas but not heart muscle. Reduced activity was not due to increased dilution or deacylation of met-tRNAmetf or to increased hydrolysis of GTP by extracts from starved animals.",

author = "{Eugene Rannels}, D. and Pegg, {Anthony E.} and Rannels, {Stephen R.}",

note = "Funding Information: The authors are grateful to Dr. L. S. Jefferson and Dr. H. E. Morgan for their encouragement and financial support. This work was supported by grants HLI1534, AM15658 and CA18138 from the National Institutes of Health.",

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doi = "10.1016/S0006-291X(76)80181-7",

language = "English (US)",

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T1 - Starvation results in decreased initiation factor activity in rat skeletal muscle

AU - Eugene Rannels, D.

AU - Pegg, Anthony E.

AU - Rannels, Stephen R.

N1 - Funding Information: The authors are grateful to Dr. L. S. Jefferson and Dr. H. E. Morgan for their encouragement and financial support. This work was supported by grants HLI1534, AM15658 and CA18138 from the National Institutes of Health.

PY - 1976/10/18

Y1 - 1976/10/18

N2 - Activity of a factor forming a nitrocellulose filter-bound complex with [35S]met-tRNAmetf and GTP was estimated in the post-ribosomal supernatant of psoas and heart muscle. Complex formation required initiator tRNA and GTP or GMP-P(CH2)P; it was inhibited by Mg2+, GDP, aurintricarboxylic acid, spermine and spermidine. Starvation reduced complex-forming activity of supernatants from psoas but not heart muscle. Reduced activity was not due to increased dilution or deacylation of met-tRNAmetf or to increased hydrolysis of GTP by extracts from starved animals.

AB - Activity of a factor forming a nitrocellulose filter-bound complex with [35S]met-tRNAmetf and GTP was estimated in the post-ribosomal supernatant of psoas and heart muscle. Complex formation required initiator tRNA and GTP or GMP-P(CH2)P; it was inhibited by Mg2+, GDP, aurintricarboxylic acid, spermine and spermidine. Starvation reduced complex-forming activity of supernatants from psoas but not heart muscle. Reduced activity was not due to increased dilution or deacylation of met-tRNAmetf or to increased hydrolysis of GTP by extracts from starved animals.

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ER -

Starvation results in decreased initiation factor activity in rat skeletal muscle

Abstract

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