Activity of a factor forming a nitrocellulose filter-bound complex with [35S]met-tRNAmetf and GTP was estimated in the post-ribosomal supernatant of psoas and heart muscle. Complex formation required initiator tRNA and GTP or GMP-P(CH2)P; it was inhibited by Mg2+, GDP, aurintricarboxylic acid, spermine and spermidine. Starvation reduced complex-forming activity of supernatants from psoas but not heart muscle. Reduced activity was not due to increased dilution or deacylation of met-tRNAmetf or to increased hydrolysis of GTP by extracts from starved animals.
|Number of pages
|Biochemical and Biophysical Research Communications
|Published - Oct 18 1976
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology