Stereoselective separation and detection of phenoxy acid herbicide enantiomers by cyclodextrin-modified capillary zone electrophoresis-electrospray ionization mass spectrometry

Koji Otsuka, Christopher J. Smith, James Grainger, John R. Barr, Donald G. Patterson, Nobuo Tanaka, Shigeru Terabe

Research output: Contribution to journalArticlepeer-review

65 Scopus citations

Abstract

An application of on-line coupling of capillary electrophoresis and mass spectrometry (CE-MS) to the chiral separation of phenoxy acid herbicide enantiomers was investigated. As an ionization method, electrospray ionization (ESI) is used for a CE-MS interface. Generally, nonvolatile additives in separation solutions sometimes decrease the MS sensitivity and/or signal intensity. In this study, however, heptakis(2,3,6-tri-O-methyl)-β-cyclodextrin (TM-β-CD) was used as a chiral selector and it migrated directly into the ESI interface. By using the negative-ionization mode along with a methanol-water-formic acid solution as a sheath liquid and nitrogen as a sheath gas, stereoselective separation and detection of three phenoxy acid herbicide enantiomers were successfully achieved with a 20 mM TM-β-CD in a 50 mM ammonium acetate buffer (pH 4.6). Copyright (C) 1998 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)75-81
Number of pages7
JournalJournal of Chromatography A
Volume817
Issue number1-2
DOIs
StatePublished - Aug 21 1998

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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