The potential of lung tissue of adult male rats to metabolize dihydrotestosterone (DHT) in vitro was examined. Within 3 min, a homogenate of 100 mg lung tissue, cleared of blood by perfusion before homogenization, metabolized 90% of the [3H]DHT substrate. Approximately 80% of the DHT was converted to 5α-androstan-3α, 17β-diol. The amount of 5α-androstan- 3α, 17β-diol formed during a 5-min incubation increased linearly, with substrate concentrations ranging from 3.3 × 10-8 to 3 × 10-6 M. Thus, the capacity of rat lung tissue to metabolize DHT in vitro and the rate of 3α-reduction of DHT are sufficiently great to consider the possibility that lung may be responsible for the rapid clearance of DHT from the circulation in this species.
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