Abstract
Stimulated Raman scattering (SRS) microscopy that is capable of both high-speed imaging and rapid spectroscopy will be advantageous for detailed chemical analysis of heterogeneous biological specimens. We have developed a system based on spectral focusing SRS technology with the integration of a rapid scanning optical delay line, which allows continuous tuning of SRS spectra by scanning a galvo mirror. We demonstrate SRS spectral measurements of dimethyl sulfoxide solution at low concentrations and multi-color imaging of rice pollens and HeLa cells with line-by-line delay tuning to reduce motion artifacts, as well as fast acquisition of SRS spectra at specific regions of interest.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 659-662 |
| Number of pages | 4 |
| Journal | Optics Letters |
| Volume | 42 |
| Issue number | 4 |
| DOIs | |
| State | Published - Feb 15 2017 |
All Science Journal Classification (ASJC) codes
- Atomic and Molecular Physics, and Optics