Strain and plastic composite support (PCS) selection for vitamin K (Menaquinone-7) production in biofilm reactors

Ehsan Mahdinia, Ali Demirci, Aydin Berenjian

Research output: Contribution to journalArticlepeer-review

35 Scopus citations


Menaquinone-7 (MK-7), a subtype of vitamin K, has received a significant attention due to its effect on improving bone and cardiovascular health. Current fermentation strategies, which involve static fermentation without aeration or agitation, are associated with low productivity and scale-up issues and hardly justify the commercial production needs of this vitamin. Previous studies indicate that static fermentation is associated with pellicle and biofilm formations, which are critical for MK-7 secretion while posing significant operational issues. Therefore, the present study is undertaken to evaluate the possibility of using a biofilm reactor as a new strategy for MK-7 fermentation. Bacillus species, namely, Bacillus subtilis natto, Bacillus licheniformis, and Bacillus amyloliquifaciens as well as plastic composite, supports (PCS) were investigated in terms of MK-7 production and biofilm formation. Results show the possibility of using a biofilm reactor for MK-7 biosynthesis. Bacillus subtilis natto and soybean flour yeast extract PCS in glucose medium were found as the most potent combination for production of MK-7 as high as 35.5 mg/L, which includes both intracellular and extracellular MK-7.

Original languageEnglish (US)
Pages (from-to)1507-1517
Number of pages11
JournalBioprocess and biosystems engineering
Issue number10
StatePublished - Oct 2017

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering


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