Streptomyces-derived actinomycin D inhibits biofilm formation by Staphylococcus aureus and its hemolytic activity

Jin Hyung Lee, Yong Guy Kim, Kayeon Lee, Chang Jin Kim, Dong Jin Park, Yoonjung Ju, Jae Chan Lee, Thomas K. Wood, Jintae Lee

Research output: Contribution to journalArticlepeer-review

33 Scopus citations


Staphylococcus aureus is a versatile human pathogen that produces diverse virulence factors, and its biofilm cells are difficult to eradicate due to their inherent ability to tolerate antibiotics. The anti-biofilm activities of the spent media of 252 diverse endophytic microorganisms were investigated using three S. aureus strains. An attempt was made to identify anti-biofilm compounds in active spent media and to assess their anti-hemolytic activities and hydrophobicities in order to investigate action mechanisms. Unlike other antibiotics, actinomycin D (0.5 μg ml−1) from Streptomyces parvulus significantly inhibited biofilm formation by all three S. aureus strains. Actinomycin D inhibited slime production in S. aureus and it inhibited hemolysis by S. aureus and caused S. aureus cells to become less hydrophobic, thus supporting its anti-biofilm effect. In addition, surface coatings containing actinomycin D prevented S. aureus biofilm formation on glass surfaces. Given these results, FDA-approved actinomycin D warrants further attention as a potential antivirulence agent against S. aureus infections.

Original languageEnglish (US)
Pages (from-to)45-56
Number of pages12
Issue number1
StatePublished - Jan 2 2016

All Science Journal Classification (ASJC) codes

  • Aquatic Science
  • Applied Microbiology and Biotechnology
  • Water Science and Technology


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