Streptomyces lividans glycosylates an exoglucanase (Cex) from Cellulomonas fimi

Alasdair M. MacLeod; Neil R. Gilkes; Loida Escote-Carlson; R. Antony; J. Warren; Douglas G. Kilburn; Robert C. Miller

doi:10.1016/0378-1119(92)90173-M

Streptomyces lividans glycosylates an exoglucanase (Cex) from Cellulomonas fimi

Alasdair M. MacLeod, Neil R. Gilkes, Loida Escote-Carlson, R. Antony, J. Warren, Douglas G. Kilburn, Robert C. Miller

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Exoglucanase Cex from Cellulomonas fimi is a glycoprotein [Langsford et al., J. Gen. Microbiol. 130 (1984) 1367-1376]. Cex produced by Streptomyces lividans from the cloned cex gene is also glycosylated. The extent and nature of glycosylation are similar for Cex from both organisms. The glycosylation affords protection against proteolysis for the enzymes from both organisms when they are bound to cellulose, but not in solution. The ability to glycosylate cloned gene products enhances the utility of Streptomyces as a host for the production of heterologous polypeptides.

Original language	English (US)
Pages (from-to)	143-147
Number of pages	5
Journal	Gene
Volume	121
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(92)90173-M
State	Published - Nov 2 1992

All Science Journal Classification (ASJC) codes

Genetics

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10.1016/0378-1119(92)90173-M

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title = "Streptomyces lividans glycosylates an exoglucanase (Cex) from Cellulomonas fimi",

abstract = "Exoglucanase Cex from Cellulomonas fimi is a glycoprotein [Langsford et al., J. Gen. Microbiol. 130 (1984) 1367-1376]. Cex produced by Streptomyces lividans from the cloned cex gene is also glycosylated. The extent and nature of glycosylation are similar for Cex from both organisms. The glycosylation affords protection against proteolysis for the enzymes from both organisms when they are bound to cellulose, but not in solution. The ability to glycosylate cloned gene products enhances the utility of Streptomyces as a host for the production of heterologous polypeptides.",

author = "MacLeod, {Alasdair M.} and Gilkes, {Neil R.} and Loida Escote-Carlson and R. Antony and J. Warren and Kilburn, {Douglas G.} and Miller, {Robert C.}",

note = "Funding Information: Streptomyces strains and plasmids were kindly provided by D.A. Hopwood. This work was supported by the Natural Sciences and Engineering Research Council of Canada. A.M.MacL. was supported by a postgraduate scholarship from the Council.",

year = "1992",

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doi = "10.1016/0378-1119(92)90173-M",

language = "English (US)",

volume = "121",

pages = "143--147",

journal = "Gene",

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TY - JOUR

T1 - Streptomyces lividans glycosylates an exoglucanase (Cex) from Cellulomonas fimi

AU - MacLeod, Alasdair M.

AU - Gilkes, Neil R.

AU - Escote-Carlson, Loida

AU - Antony, R.

AU - Warren, J.

AU - Kilburn, Douglas G.

AU - Miller, Robert C.

N1 - Funding Information: Streptomyces strains and plasmids were kindly provided by D.A. Hopwood. This work was supported by the Natural Sciences and Engineering Research Council of Canada. A.M.MacL. was supported by a postgraduate scholarship from the Council.

PY - 1992/11/2

Y1 - 1992/11/2

N2 - Exoglucanase Cex from Cellulomonas fimi is a glycoprotein [Langsford et al., J. Gen. Microbiol. 130 (1984) 1367-1376]. Cex produced by Streptomyces lividans from the cloned cex gene is also glycosylated. The extent and nature of glycosylation are similar for Cex from both organisms. The glycosylation affords protection against proteolysis for the enzymes from both organisms when they are bound to cellulose, but not in solution. The ability to glycosylate cloned gene products enhances the utility of Streptomyces as a host for the production of heterologous polypeptides.

AB - Exoglucanase Cex from Cellulomonas fimi is a glycoprotein [Langsford et al., J. Gen. Microbiol. 130 (1984) 1367-1376]. Cex produced by Streptomyces lividans from the cloned cex gene is also glycosylated. The extent and nature of glycosylation are similar for Cex from both organisms. The glycosylation affords protection against proteolysis for the enzymes from both organisms when they are bound to cellulose, but not in solution. The ability to glycosylate cloned gene products enhances the utility of Streptomyces as a host for the production of heterologous polypeptides.

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JO - Gene

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ER -

Streptomyces lividans glycosylates an exoglucanase (Cex) from Cellulomonas fimi

Abstract

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