TY - JOUR
T1 - Structural Basis of DNA Recognition by the Alternative Sigma-factor, σ54
AU - Doucleff, Michaeleen
AU - Pelton, Jeffrey G.
AU - Lee, Peter S.
AU - Nixon, B. Tracy
AU - Wemmer, David E.
N1 - Funding Information:
We thank Dr Ann E. Maris for help with the DNA-binding assays and Dr Junji Iwahara for help with protein–DNA docking. This work was supported by National Institutes of Health grant GM62163 (to D. E. W.) and by a University of California fellowship (to M. D.). In addition, National Science Foundation grants BBS8720134, 0119304 (to D. E. W.) and National Institutes of Health grant RR15756 (to D. E. W.) provided support for the 600 and 800 Mhz spectrometers used in this research.
PY - 2007/6/15
Y1 - 2007/6/15
N2 - The σ subunit of bacterial RNA polymerase (RNAP) regulates gene expression by directing RNAP to specific promoters. Unlike σ70-type proteins, the alternative σ factor, σ54, requires interaction with an ATPase to open DNA. We present the solution structure of the C-terminal domain of σ54 bound to the -24 promoter element, in which the conserved RpoN box motif inserts into the major groove of the DNA. This structure elucidates the basis for sequence specific recognition of the -24 element, orients σ54 on the promoter, and suggests how the C-terminal domain of σ54 interacts with RNAP.
AB - The σ subunit of bacterial RNA polymerase (RNAP) regulates gene expression by directing RNAP to specific promoters. Unlike σ70-type proteins, the alternative σ factor, σ54, requires interaction with an ATPase to open DNA. We present the solution structure of the C-terminal domain of σ54 bound to the -24 promoter element, in which the conserved RpoN box motif inserts into the major groove of the DNA. This structure elucidates the basis for sequence specific recognition of the -24 element, orients σ54 on the promoter, and suggests how the C-terminal domain of σ54 interacts with RNAP.
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U2 - 10.1016/j.jmb.2007.04.019
DO - 10.1016/j.jmb.2007.04.019
M3 - Article
C2 - 17481658
AN - SCOPUS:34248578701
SN - 0022-2836
VL - 369
SP - 1070
EP - 1078
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
IS - 4
ER -