@article{d475671bcae64a7dbb84970ac76a94cf,
title = "Structural characterization of NRAS isoform 5",
abstract = "It was recently discovered that the NRAS isoform 5 (20 amino acids) is expressed in melanoma and results in a more aggressive cell phenotype. This novel isoform is responsible for increased phosphorylation of downstream targets such as AKT, MEK, and ERK as well as increased cellular proliferation. This structure report describes the NMR solution structure of NRAS isoform 5 to be used as a starting point to understand its biophysical interactions. The isoform is highly flexible in aqueous solution, but forms a helix-turn-coil structure in the presence of trifluoroethanol as determined by NMR and CD spectroscopy.",
author = "Joseph Markowitz and Mal, {Tapas K.} and Chunhua Yuan and Courtney, {Nicholas B.} and Mitra Patel and Stiff, {Andrew R.} and James Blachly and Christopher Walker and Eisfeld, {Ann Kathrin} and {De La Chapelle}, Albert and Carson, {William E.}",
note = "Funding Information: Authors acknowledge Marina Bakhtina, Ph.D. for her technical assistance on the Jasco CD spectrometer in the OSU Biophysical Interaction and Characterization Facility and Isaac P. Foote for his assistance in producing the circular dichroism figures for this paper. Any opinions, findings, and conclusions expressed in this material are those of the author(s) and do not necessarily reflect those of the Pelotonia Fellowship Program. J. Markowitz is currently supported by the Donald A. Adam Comprehensive Melanoma Research Center at Moffitt Cancer Center. Publisher Copyright: {\textcopyright} 2016 The Protein Society.",
year = "2016",
month = may,
day = "1",
doi = "10.1002/pro.2916",
language = "English (US)",
volume = "25",
pages = "1069--1074",
journal = "Protein Science",
issn = "0961-8368",
publisher = "Cold Spring Harbor Laboratory Press",
number = "5",
}