Structural Composition of Canine Secretory Component and Immunoglobulin A

Russell E. Thompson; Herbert Y. Reynolds; Myron J. Waxdal

doi:10.1021/bi00684a010

Structural Composition of Canine Secretory Component and Immunoglobulin A

Russell E. Thompson, Herbert Y. Reynolds, Myron J. Waxdal

Department of Medicine

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Dog serum and colostral immunoglobulin A (IgA) and free secretory component from colostrum were isolated using affinity chromatography. Both serum and colostral IgA showed similar susceptibility to reduction with dithiothreitol, but only colostral IgA released the additional subunit, bound secretory component. This released secretory component was identical with free secretory component with respect to electrophoretic migration, isoelectric focusing point, and molecular weight, but lacked some antigenic determinants. The amino acid composition and the N-terminal sequence of canine free secretory component was similar to that reported for the human and cow.

Original language	English (US)
Pages (from-to)	2853-2860
Number of pages	8
Journal	Biochemistry
Volume	14
Issue number	13
DOIs	https://doi.org/10.1021/bi00684a010
State	Published - Jul 1 1975

All Science Journal Classification (ASJC) codes

Biochemistry

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10.1021/bi00684a010

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abstract = "Dog serum and colostral immunoglobulin A (IgA) and free secretory component from colostrum were isolated using affinity chromatography. Both serum and colostral IgA showed similar susceptibility to reduction with dithiothreitol, but only colostral IgA released the additional subunit, bound secretory component. This released secretory component was identical with free secretory component with respect to electrophoretic migration, isoelectric focusing point, and molecular weight, but lacked some antigenic determinants. The amino acid composition and the N-terminal sequence of canine free secretory component was similar to that reported for the human and cow.",

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year = "1975",

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AU - Reynolds, Herbert Y.

AU - Waxdal, Myron J.

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N2 - Dog serum and colostral immunoglobulin A (IgA) and free secretory component from colostrum were isolated using affinity chromatography. Both serum and colostral IgA showed similar susceptibility to reduction with dithiothreitol, but only colostral IgA released the additional subunit, bound secretory component. This released secretory component was identical with free secretory component with respect to electrophoretic migration, isoelectric focusing point, and molecular weight, but lacked some antigenic determinants. The amino acid composition and the N-terminal sequence of canine free secretory component was similar to that reported for the human and cow.

AB - Dog serum and colostral immunoglobulin A (IgA) and free secretory component from colostrum were isolated using affinity chromatography. Both serum and colostral IgA showed similar susceptibility to reduction with dithiothreitol, but only colostral IgA released the additional subunit, bound secretory component. This released secretory component was identical with free secretory component with respect to electrophoretic migration, isoelectric focusing point, and molecular weight, but lacked some antigenic determinants. The amino acid composition and the N-terminal sequence of canine free secretory component was similar to that reported for the human and cow.

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JO - Biochemistry

JF - Biochemistry

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ER -

Structural Composition of Canine Secretory Component and Immunoglobulin A

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