TY - JOUR
T1 - Structural organization and transcription of the mouse gastric H+,K+-ATPase β subunit gene
AU - Canfield, V. A.
AU - Levenson, R.
PY - 1991
Y1 - 1991
N2 - We have cloned and characterized the mouse gene encoding the β subunit of H+,K+-ATPase (EC 3.6.1.36). The entire 10.5-kilobase transcription unit of the H+,K+-ATPase β subunit gene was cloned in three overlapping cosmids encompassing ≈46 kilobases of genomic DNA. A tight cluster of transcription initiation sites has been localized 24-25 nucleotides upstream of the translation start site and 28-29 nucleotides downstream of a TATA-like sequence. The H+,K+-ATPase β subunit gene is split into seven exons encoding predicted structural domains of the β subunit protein. The intracellular amino-terminal and putative transmembrane domains are encoded by individual exons, and the extracellular carboxyl-terminal domain is encoded by five exons. The exon/intron organization of the mouse H+,K+-ATPase β subunit gene is identical to that of the mouse Na+,K+-ATPase β2 subunit gene. The conservation of genomic organization, together with the high sequence homotogy, indicates that the noose H+,K+-ATPase β and Na+,K+-ATPase β2 subunit genes originated from a common ancestral gene.
AB - We have cloned and characterized the mouse gene encoding the β subunit of H+,K+-ATPase (EC 3.6.1.36). The entire 10.5-kilobase transcription unit of the H+,K+-ATPase β subunit gene was cloned in three overlapping cosmids encompassing ≈46 kilobases of genomic DNA. A tight cluster of transcription initiation sites has been localized 24-25 nucleotides upstream of the translation start site and 28-29 nucleotides downstream of a TATA-like sequence. The H+,K+-ATPase β subunit gene is split into seven exons encoding predicted structural domains of the β subunit protein. The intracellular amino-terminal and putative transmembrane domains are encoded by individual exons, and the extracellular carboxyl-terminal domain is encoded by five exons. The exon/intron organization of the mouse H+,K+-ATPase β subunit gene is identical to that of the mouse Na+,K+-ATPase β2 subunit gene. The conservation of genomic organization, together with the high sequence homotogy, indicates that the noose H+,K+-ATPase β and Na+,K+-ATPase β2 subunit genes originated from a common ancestral gene.
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M3 - Article
C2 - 1654563
AN - SCOPUS:0025858464
SN - 0027-8424
VL - 88
SP - 8247
EP - 8251
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 18
ER -