Structural studies of an acidic polysaccharide from Ocimum basilicum seeds

Yernool V. Anjaneyalu; D. Channe Gowda

doi:10.1016/S0008-6215(00)84644-3

Structural studies of an acidic polysaccharide from Ocimum basilicum seeds

Yernool V. Anjaneyalu, D. Channe Gowda

Department of Biochemistry and Molecular Biology

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48 Scopus citations

Abstract

An acidic polysaccharide isolated from the seeds of Ocimum basilicum by DEAE-cellulose fractionation was ∼92% pure, having an associated glucan impurity (∼8%). The polysaccharide is composed of d-xylose, l-arabinose, l-rhamnose, and d-galacturonic acid in the molar ratios 15:9:7:12, together with traces or galactose and glucose. Methylation analysis indicated that the polysaccharide contained a (1→4)-linked xylan backbone carrying branch-points at C-2 and C-3 of the xylosyl residues, and revealed the structural features of the side chains. Periodateoxidation and Smith-degradation studies support the results of methylation analysis.

Original language	English (US)
Pages (from-to)	251-256
Number of pages	6
Journal	Carbohydrate Research
Volume	75
Issue number	C
DOIs	https://doi.org/10.1016/S0008-6215(00)84644-3
State	Published - Oct 1979

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Biochemistry
Organic Chemistry

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abstract = "An acidic polysaccharide isolated from the seeds of Ocimum basilicum by DEAE-cellulose fractionation was ∼92% pure, having an associated glucan impurity (∼8%). The polysaccharide is composed of d-xylose, l-arabinose, l-rhamnose, and d-galacturonic acid in the molar ratios 15:9:7:12, together with traces or galactose and glucose. Methylation analysis indicated that the polysaccharide contained a (1→4)-linked xylan backbone carrying branch-points at C-2 and C-3 of the xylosyl residues, and revealed the structural features of the side chains. Periodateoxidation and Smith-degradation studies support the results of methylation analysis.",

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T1 - Structural studies of an acidic polysaccharide from Ocimum basilicum seeds

AU - Anjaneyalu, Yernool V.

AU - Gowda, D. Channe

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N2 - An acidic polysaccharide isolated from the seeds of Ocimum basilicum by DEAE-cellulose fractionation was ∼92% pure, having an associated glucan impurity (∼8%). The polysaccharide is composed of d-xylose, l-arabinose, l-rhamnose, and d-galacturonic acid in the molar ratios 15:9:7:12, together with traces or galactose and glucose. Methylation analysis indicated that the polysaccharide contained a (1→4)-linked xylan backbone carrying branch-points at C-2 and C-3 of the xylosyl residues, and revealed the structural features of the side chains. Periodateoxidation and Smith-degradation studies support the results of methylation analysis.

AB - An acidic polysaccharide isolated from the seeds of Ocimum basilicum by DEAE-cellulose fractionation was ∼92% pure, having an associated glucan impurity (∼8%). The polysaccharide is composed of d-xylose, l-arabinose, l-rhamnose, and d-galacturonic acid in the molar ratios 15:9:7:12, together with traces or galactose and glucose. Methylation analysis indicated that the polysaccharide contained a (1→4)-linked xylan backbone carrying branch-points at C-2 and C-3 of the xylosyl residues, and revealed the structural features of the side chains. Periodateoxidation and Smith-degradation studies support the results of methylation analysis.

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Structural studies of an acidic polysaccharide from Ocimum basilicum seeds

Abstract

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