TY - JOUR
T1 - Structure and function of lineage-specific sequence insertions in the bacterial RNA polymerase β′ subunit
AU - Chlenov, Mark
AU - Masuda, Shoko
AU - Murakami, Katsuhiko S.
AU - Nikiforov, Vadim
AU - Darst, Seth A.
AU - Mustaev, Arkady
N1 - Funding Information:
We thank Elena Severinova and Konstantin Severinov for allowing us to cite unpublished data, and Dmitry G. Vassylyev for discussion involving the Thermus β′NCD structure. We are indebted to the staff at the Cornell High Energy Sychrotron Source, and at the National Synchrotron Light Source beamlines X9A and X9B, for support during data collection. K.S.M. was supported by a Norman and Rosita Winston Postdoctoral Fellowship and a Human Frontier Science Program Postdoctoral Fellowship. Supported in part by NIH grants GM30717 to Alex Goldfarb, and GM61898 to S.A.D.
PY - 2005/10/14
Y1 - 2005/10/14
N2 - The large β and β′ subunits of the bacterial core RNA polymerase (RNAP) are highly conserved throughout evolution. Nevertheless, large sequence insertions in β and β′ characterize specific evolutionary lineages of bacteria. The Thermus aquaticus RNAP β′ subunit contains a 283 residue insert between conserved regions A and B that is found in only four bacterial species. The Escherichia coli RNAP β′ subunit contains a 188 residue insert in the middle of conserved region G that is found in a wide range of bacterial species. Here, we present structural studies of these two β′ insertions. We show that the inserts comprise repeats of a previously characterized fold, the sandwich-barrel hybrid motif (as predicted from previous sequence analysis) and that the inserts serve significant roles in facilitating protein/protein and/or protein/nucleic acid interactions.
AB - The large β and β′ subunits of the bacterial core RNA polymerase (RNAP) are highly conserved throughout evolution. Nevertheless, large sequence insertions in β and β′ characterize specific evolutionary lineages of bacteria. The Thermus aquaticus RNAP β′ subunit contains a 283 residue insert between conserved regions A and B that is found in only four bacterial species. The Escherichia coli RNAP β′ subunit contains a 188 residue insert in the middle of conserved region G that is found in a wide range of bacterial species. Here, we present structural studies of these two β′ insertions. We show that the inserts comprise repeats of a previously characterized fold, the sandwich-barrel hybrid motif (as predicted from previous sequence analysis) and that the inserts serve significant roles in facilitating protein/protein and/or protein/nucleic acid interactions.
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U2 - 10.1016/j.jmb.2005.07.073
DO - 10.1016/j.jmb.2005.07.073
M3 - Article
C2 - 16154587
AN - SCOPUS:25144510935
SN - 0022-2836
VL - 353
SP - 138
EP - 154
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
IS - 1
ER -