Structure of antibacterial peptide microcin J25: A 21-residue lariat protoknot

Marvin J. Bayro; Jayanta Mukhopadhyay; G. V.T. Swapna; Janet Y. Huang; Li Chung Ma; Elena Sineva; Philip E. Dawson; Gaetano T. Montelione; Richard H. Ebright

doi:10.1021/ja036677e

Structure of antibacterial peptide microcin J25: A 21-residue lariat protoknot

Marvin J. Bayro, Jayanta Mukhopadhyay, G. V.T. Swapna, Janet Y. Huang, Li Chung Ma, Elena Sineva, Philip E. Dawson, Gaetano T. Montelione, Richard H. Ebright

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › peer-review

189 Scopus citations

Abstract

The antibacterial peptide microcin J25 (MccJ25) inhibits bacterial transcription by binding within, and obstructing, the nucleotide-uptake channel of bacterial RNA polymerase. Published covalent and three-dimensional structures indicate that MccJ25 is a 21-residue cycle. Here, we show that the published covalent and three-dimensional structures are incorrect, and that MccJ25 in fact is a 21-residue "lariat protoknot", consisting of an 8-residue cyclic segment followed by a 13-residue linear segment that loops back and threads through the cyclic segment. MccJ25 is the first example of a lariat protoknot involving a backbone-side chain amide linkage.

Original language	English (US)
Pages (from-to)	12382-12383
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	125
Issue number	41
DOIs	https://doi.org/10.1021/ja036677e
State	Published - Oct 15 2003

All Science Journal Classification (ASJC) codes

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

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title = "Structure of antibacterial peptide microcin J25: A 21-residue lariat protoknot",

abstract = "The antibacterial peptide microcin J25 (MccJ25) inhibits bacterial transcription by binding within, and obstructing, the nucleotide-uptake channel of bacterial RNA polymerase. Published covalent and three-dimensional structures indicate that MccJ25 is a 21-residue cycle. Here, we show that the published covalent and three-dimensional structures are incorrect, and that MccJ25 in fact is a 21-residue {"}lariat protoknot{"}, consisting of an 8-residue cyclic segment followed by a 13-residue linear segment that loops back and threads through the cyclic segment. MccJ25 is the first example of a lariat protoknot involving a backbone-side chain amide linkage.",

author = "Bayro, {Marvin J.} and Jayanta Mukhopadhyay and Swapna, {G. V.T.} and Huang, {Janet Y.} and Ma, {Li Chung} and Elena Sineva and Dawson, {Philip E.} and Montelione, {Gaetano T.} and Ebright, {Richard H.}",

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TY - JOUR

T1 - Structure of antibacterial peptide microcin J25

T2 - A 21-residue lariat protoknot

AU - Bayro, Marvin J.

AU - Mukhopadhyay, Jayanta

AU - Swapna, G. V.T.

AU - Huang, Janet Y.

AU - Ma, Li Chung

AU - Sineva, Elena

AU - Dawson, Philip E.

AU - Montelione, Gaetano T.

AU - Ebright, Richard H.

PY - 2003/10/15

Y1 - 2003/10/15

N2 - The antibacterial peptide microcin J25 (MccJ25) inhibits bacterial transcription by binding within, and obstructing, the nucleotide-uptake channel of bacterial RNA polymerase. Published covalent and three-dimensional structures indicate that MccJ25 is a 21-residue cycle. Here, we show that the published covalent and three-dimensional structures are incorrect, and that MccJ25 in fact is a 21-residue "lariat protoknot", consisting of an 8-residue cyclic segment followed by a 13-residue linear segment that loops back and threads through the cyclic segment. MccJ25 is the first example of a lariat protoknot involving a backbone-side chain amide linkage.

AB - The antibacterial peptide microcin J25 (MccJ25) inhibits bacterial transcription by binding within, and obstructing, the nucleotide-uptake channel of bacterial RNA polymerase. Published covalent and three-dimensional structures indicate that MccJ25 is a 21-residue cycle. Here, we show that the published covalent and three-dimensional structures are incorrect, and that MccJ25 in fact is a 21-residue "lariat protoknot", consisting of an 8-residue cyclic segment followed by a 13-residue linear segment that loops back and threads through the cyclic segment. MccJ25 is the first example of a lariat protoknot involving a backbone-side chain amide linkage.

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Structure of antibacterial peptide microcin J25: A 21-residue lariat protoknot

Abstract

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