TY - JOUR
T1 - Structure of Escherichia coli ribonucleotide reductase R2 in space group P6122
AU - Sommerhalter, Monika
AU - Saleh, Lana
AU - Bollinger, J. Martin
AU - Rosenzweig, Amy C.
PY - 2005/12
Y1 - 2005/12
N2 - A new crystal form of wild-type ribonucleotide reductase R2 from Escherichia coli was obtained. Crystals grow in space group P6122 with one R2 monomer in the asymmetric unit. A twofold crystallographic symmetry axis generates the physiological dimeric form of R2. Co-crystallization with CoCl2 or MnCl2 results in full occupancy of the dinuclear metal site. The structure of the MnII-loaded form was determined to 2.6 Å resolution by molecular replacement. The crystallization conditions, backbone conformation, crystal-packing interactions and metal centers are compared with those of previously determined crystal forms.
AB - A new crystal form of wild-type ribonucleotide reductase R2 from Escherichia coli was obtained. Crystals grow in space group P6122 with one R2 monomer in the asymmetric unit. A twofold crystallographic symmetry axis generates the physiological dimeric form of R2. Co-crystallization with CoCl2 or MnCl2 results in full occupancy of the dinuclear metal site. The structure of the MnII-loaded form was determined to 2.6 Å resolution by molecular replacement. The crystallization conditions, backbone conformation, crystal-packing interactions and metal centers are compared with those of previously determined crystal forms.
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U2 - 10.1107/S0907444905034062
DO - 10.1107/S0907444905034062
M3 - Article
C2 - 16301799
AN - SCOPUS:33644848547
SN - 0907-4449
VL - 61
SP - 1649
EP - 1654
JO - Acta Crystallographica Section D: Biological Crystallography
JF - Acta Crystallographica Section D: Biological Crystallography
IS - 12
ER -