Structure of Escherichia coli ribonucleotide reductase R2 in space group P6122

Monika Sommerhalter; Lana Saleh; J. Martin Bollinger; Amy C. Rosenzweig

doi:10.1107/S0907444905034062

Structure of Escherichia coli ribonucleotide reductase R2 in space group P6₁22

Monika Sommerhalter
, Lana Saleh
, J. Martin Bollinger
, Amy C. Rosenzweig

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

A new crystal form of wild-type ribonucleotide reductase R2 from Escherichia coli was obtained. Crystals grow in space group P6₁22 with one R2 monomer in the asymmetric unit. A twofold crystallographic symmetry axis generates the physiological dimeric form of R2. Co-crystallization with CoCl₂ or MnCl₂ results in full occupancy of the dinuclear metal site. The structure of the Mn^II-loaded form was determined to 2.6 Å resolution by molecular replacement. The crystallization conditions, backbone conformation, crystal-packing interactions and metal centers are compared with those of previously determined crystal forms.

Original language	English (US)
Pages (from-to)	1649-1654
Number of pages	6
Journal	Acta Crystallographica Section D: Biological Crystallography
Volume	61
Issue number	12
DOIs	https://doi.org/10.1107/S0907444905034062
State	Published - Dec 2005

All Science Journal Classification (ASJC) codes

Structural Biology

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10.1107/S0907444905034062

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abstract = "A new crystal form of wild-type ribonucleotide reductase R2 from Escherichia coli was obtained. Crystals grow in space group P6122 with one R2 monomer in the asymmetric unit. A twofold crystallographic symmetry axis generates the physiological dimeric form of R2. Co-crystallization with CoCl2 or MnCl2 results in full occupancy of the dinuclear metal site. The structure of the MnII-loaded form was determined to 2.6 {\AA} resolution by molecular replacement. The crystallization conditions, backbone conformation, crystal-packing interactions and metal centers are compared with those of previously determined crystal forms.",

author = "Monika Sommerhalter and Lana Saleh and Bollinger, \{J. Martin\} and Rosenzweig, \{Amy C.\}",

year = "2005",

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language = "English (US)",

volume = "61",

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journal = "Acta Crystallographica Section D: Biological Crystallography",

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T1 - Structure of Escherichia coli ribonucleotide reductase R2 in space group P6122

AU - Sommerhalter, Monika

AU - Saleh, Lana

AU - Bollinger, J. Martin

AU - Rosenzweig, Amy C.

PY - 2005/12

Y1 - 2005/12

N2 - A new crystal form of wild-type ribonucleotide reductase R2 from Escherichia coli was obtained. Crystals grow in space group P6122 with one R2 monomer in the asymmetric unit. A twofold crystallographic symmetry axis generates the physiological dimeric form of R2. Co-crystallization with CoCl2 or MnCl2 results in full occupancy of the dinuclear metal site. The structure of the MnII-loaded form was determined to 2.6 Å resolution by molecular replacement. The crystallization conditions, backbone conformation, crystal-packing interactions and metal centers are compared with those of previously determined crystal forms.

AB - A new crystal form of wild-type ribonucleotide reductase R2 from Escherichia coli was obtained. Crystals grow in space group P6122 with one R2 monomer in the asymmetric unit. A twofold crystallographic symmetry axis generates the physiological dimeric form of R2. Co-crystallization with CoCl2 or MnCl2 results in full occupancy of the dinuclear metal site. The structure of the MnII-loaded form was determined to 2.6 Å resolution by molecular replacement. The crystallization conditions, backbone conformation, crystal-packing interactions and metal centers are compared with those of previously determined crystal forms.

UR - https://www.scopus.com/pages/publications/33644848547

UR - https://www.scopus.com/pages/publications/33644848547#tab=citedBy

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EP - 1654

JO - Acta Crystallographica Section D: Biological Crystallography

JF - Acta Crystallographica Section D: Biological Crystallography

IS - 12

ER -

Structure of Escherichia coli ribonucleotide reductase R2 in space group P6₁22

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