@inbook{625d38d2f771458e997884bdd8f4bb1b,
title = "Structure-seq of tRNAs and other short RNAs in droplets and in vivo",
abstract = "There is a multitude of small (<100 nt) RNAs that serve diverse functional roles in biology. Key amongst these is transfer RNA (tRNA), which is among the most ancient RNAs and is part of the translational apparatus in every domain of life. Transfer RNAs are also the most heavily modified class of RNAs. They are essential and their misregulation, due to mutated sequences or loss of modification, can lead to disease. Because of the severe phenotypes associated with mitochondrial tRNA defects in particular, the desire to deliver repaired tRNAs via droplets such as lipid nanoparticles or other compartments is an active area of research. Here we describe how to use our tRNA Structure-seq method to study tRNAs and other small RNAs in two different biologically relevant contexts, peptide-rich droplets and in vivo.",
author = "Meyer, {McCauley O.} and Saehyun Choi and Keating, {Christine D.} and Bevilacqua, {Philip C.} and Ryota Yamagami",
note = "Publisher Copyright: {\textcopyright} 2023",
year = "2023",
month = jan,
doi = "10.1016/bs.mie.2023.05.006",
language = "English (US)",
isbn = "9780443157707",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
pages = "81--126",
editor = "Ryota Yamagami and Kwok, {Chun Kit} and Kwok, {Chun Kit}",
booktitle = "Enzymes in RNA Science and Biotechnology Part A",
address = "United States",
}