Structure-seq of tRNAs and other short RNAs in droplets and in vivo

McCauley O. Meyer, Saehyun Choi, Christine D. Keating, Philip C. Bevilacqua, Ryota Yamagami

Research output: Chapter in Book/Report/Conference proceedingChapter


There is a multitude of small (<100 nt) RNAs that serve diverse functional roles in biology. Key amongst these is transfer RNA (tRNA), which is among the most ancient RNAs and is part of the translational apparatus in every domain of life. Transfer RNAs are also the most heavily modified class of RNAs. They are essential and their misregulation, due to mutated sequences or loss of modification, can lead to disease. Because of the severe phenotypes associated with mitochondrial tRNA defects in particular, the desire to deliver repaired tRNAs via droplets such as lipid nanoparticles or other compartments is an active area of research. Here we describe how to use our tRNA Structure-seq method to study tRNAs and other small RNAs in two different biologically relevant contexts, peptide-rich droplets and in vivo.

Original languageEnglish (US)
Title of host publicationEnzymes in RNA Science and Biotechnology Part A
EditorsRyota Yamagami, Chun Kit Kwok, Chun Kit Kwok
PublisherAcademic Press Inc.
Number of pages46
ISBN (Print)9780443157707
StatePublished - Jan 2023

Publication series

NameMethods in Enzymology
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

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