Studies of the base composition of transfer rna and the specificity of transfer rna methylases of hamster liver

Ann Jackson; Anthony E. Pegg

doi:10.1016/0020-711X(75)90042-7

Studies of the base composition of transfer rna and the specificity of transfer rna methylases of hamster liver

Ann Jackson, Anthony E. Pegg

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

1. 1. In contrast to previous reports, examination of the base composition of tRNA preparations isolated from hamster liver showed a minor base composition similar to that of rat liver tRNA. 2. 2. tRNA methylases isolated from hamster liver tRNA were also similar to those present in rat liver extracts. 3. 3. The same methylated nucleosides were formed when unfractionated undermethylated tRNA from E. coli was used as a substrate for methylation by enzymes from either of these species. 4. 4. The same sites within the transfer RNA sequence were attacked when purified tRNA species from E. coli and yeast were used as substrates.

Original language	English (US)
Pages (from-to)	35-42
Number of pages	8
Journal	International Journal of Biochemistry
Volume	6
Issue number	1
DOIs	https://doi.org/10.1016/0020-711X(75)90042-7
State	Published - Jan 1975

All Science Journal Classification (ASJC) codes

Biochemistry

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10.1016/0020-711X(75)90042-7

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title = "Studies of the base composition of transfer rna and the specificity of transfer rna methylases of hamster liver",

abstract = "1. 1. In contrast to previous reports, examination of the base composition of tRNA preparations isolated from hamster liver showed a minor base composition similar to that of rat liver tRNA. 2. 2. tRNA methylases isolated from hamster liver tRNA were also similar to those present in rat liver extracts. 3. 3. The same methylated nucleosides were formed when unfractionated undermethylated tRNA from E. coli was used as a substrate for methylation by enzymes from either of these species. 4. 4. The same sites within the transfer RNA sequence were attacked when purified tRNA species from E. coli and yeast were used as substrates.",

author = "Ann Jackson and Pegg, {Anthony E.}",

note = "Funding Information: This research was generously supported by the Sir Michael Sobell Fellowship of the Cancer Research Campaign.",

year = "1975",

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doi = "10.1016/0020-711X(75)90042-7",

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T1 - Studies of the base composition of transfer rna and the specificity of transfer rna methylases of hamster liver

AU - Jackson, Ann

AU - Pegg, Anthony E.

N1 - Funding Information: This research was generously supported by the Sir Michael Sobell Fellowship of the Cancer Research Campaign.

PY - 1975/1

Y1 - 1975/1

N2 - 1. 1. In contrast to previous reports, examination of the base composition of tRNA preparations isolated from hamster liver showed a minor base composition similar to that of rat liver tRNA. 2. 2. tRNA methylases isolated from hamster liver tRNA were also similar to those present in rat liver extracts. 3. 3. The same methylated nucleosides were formed when unfractionated undermethylated tRNA from E. coli was used as a substrate for methylation by enzymes from either of these species. 4. 4. The same sites within the transfer RNA sequence were attacked when purified tRNA species from E. coli and yeast were used as substrates.

AB - 1. 1. In contrast to previous reports, examination of the base composition of tRNA preparations isolated from hamster liver showed a minor base composition similar to that of rat liver tRNA. 2. 2. tRNA methylases isolated from hamster liver tRNA were also similar to those present in rat liver extracts. 3. 3. The same methylated nucleosides were formed when unfractionated undermethylated tRNA from E. coli was used as a substrate for methylation by enzymes from either of these species. 4. 4. The same sites within the transfer RNA sequence were attacked when purified tRNA species from E. coli and yeast were used as substrates.

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JO - International Journal of Biochemistry

JF - International Journal of Biochemistry

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Studies of the base composition of transfer rna and the specificity of transfer rna methylases of hamster liver

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