With the novel and simple current distribution measurement gasket developed by MPFL, the performance of a single PEM fuel cell with serpentine flow field was investigated without modifying any of its components. Inhomogeneous current distributions were demonstrated clearly by the measurement results. Current distribution at different cell operating parameters, including reactant gas flow rate, reactant gas humidification temperature and cell operating temperature, were measured and the effects of such parameters were analyzed. Reactant gas flow rate exert effects on current distribution by influencing the reactant concentration distribution, while the critical effects of gas humidification and cell temperature is caused by their significant influence on water balance and water distribution in PEM fuel cell. To fully understand the combined effects of such factors, simultaneous measurement of current distribution, reactant distribution, temperature distribution and humidification distribution is necessary and will be helpful.