TY - JOUR
T1 - Study of cellular adhesion with scanning acoustic microscopy
AU - Tittmann, Bernhard R.
AU - Miyasaka, Chiaki
AU - Mastro, Andrea M.
AU - Mercer, Robyn R.
N1 - Funding Information:
Manuscript received May 5, 2006; accepted December 3, 2006. Robin R. Mercer was supported by a pre-doctoral grant from the US Army Medical Research Material Command under DAMD 17-02-1-0358. Research was supported in part by a Biotechnology Grant, Life Sciences Consortium, the Pennsylvania State University, PA Tobacco Settlement Fund, the National Foundation for Cancer Research, Center for Metastasis Research, the USMC Research University that resides at the Pennsylvania State University under Contract M67004-99-0-0037.
PY - 2007/8
Y1 - 2007/8
N2 - A mechanical scanning acoustic reflection microscope was applied to living cells (e.g., osteoblasts) to observe their undisguised shapes and to evaluate their adhesive conditions at a substrate interface. A conditioned medium was collected from a bone-metastatic breast cancer cell line, MDA-MB-231, and cultured with an immature osteoblast cell line, MC3T3-E1. To characterize the cellular adhesion, MC3T3-E1 osteoblasts were cultured with or without MDA-MB-231 conditioned medium for 2 days, then assayed with the scanning acoustic reflection microscope. At 600 MHz the scanning acoustic reflection microscope clearly indicated that MC3T3-E1 cells cultured with MDA-MB-231 conditioned medium had both an abnormal shape and poor adhesion at the substrate interface. The results are compared with those obtained with laser scanning confocal microscopy and are supported by a simple multilayer model.
AB - A mechanical scanning acoustic reflection microscope was applied to living cells (e.g., osteoblasts) to observe their undisguised shapes and to evaluate their adhesive conditions at a substrate interface. A conditioned medium was collected from a bone-metastatic breast cancer cell line, MDA-MB-231, and cultured with an immature osteoblast cell line, MC3T3-E1. To characterize the cellular adhesion, MC3T3-E1 osteoblasts were cultured with or without MDA-MB-231 conditioned medium for 2 days, then assayed with the scanning acoustic reflection microscope. At 600 MHz the scanning acoustic reflection microscope clearly indicated that MC3T3-E1 cells cultured with MDA-MB-231 conditioned medium had both an abnormal shape and poor adhesion at the substrate interface. The results are compared with those obtained with laser scanning confocal microscopy and are supported by a simple multilayer model.
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U2 - 10.1109/TUFFC.2007.420
DO - 10.1109/TUFFC.2007.420
M3 - Article
C2 - 17703653
AN - SCOPUS:34548084789
SN - 0885-3010
VL - 54
SP - 1502
EP - 1513
JO - IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control
JF - IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control
IS - 8
ER -
