The contents of the present report are focused on characterizing a thinly sectioned skin tissue with a mechanical scanning acoustic reflection microscope (tone-burst-wave mode) and describes the quantitative data acquisition technique with V(z) analysis. The reflectance function for the tissue located on a substrate was theoretically determined, and fitted into the mathematical model of the V(z) curve. The V(z) curves with frequency at 200 MHz for thinly sectioned normal and abnormal tissues located on soda-lime glasses were theoretically and experimentally formed. Their leaky surface acoustic wave velocities were obtained by the experimentally formed V(z) curves through FFT analyses. Finally, a computer simulation with a parameter-fitting technique (i.e., matching the distances of the periods of the theoretical and experimental V(z) curves by inputting different longitudinal wave velocities and densities of the tissue) was implemented to obtain the longitudinal wave velocities and densities of the tissues. The obtained longitudinal wave velocity may be used to simulate contrast analysis.