TY - JOUR
T1 - Studying human and nonhuman primate evolutionary biology with powerful in vitro and in vivo functional genomics tools
AU - Grogan, Kathleen E.
AU - Perry, George H.
N1 - Funding Information:
This work was supported by the NIH grant F32 GM123634-01 (to K.E.G.) and NIH grant R01-GM115656 (to G.H.P.). The authors would like to thank the Editor-in-Chief Jason Kamilar for the invitation to write this article, as well as the members of the Perry lab for their comments and discussion. Images were generated with the assistance of Shutterstock.
Publisher Copyright:
© 2020 Wiley Periodicals, Inc.
PY - 2020/5/1
Y1 - 2020/5/1
N2 - In recent years, tools for functional genomic studies have become increasingly feasible for use by evolutionary anthropologists. In this review, we provide brief overviews of several exciting in vitro techniques that can be paired with “-omics” approaches (e.g., genomics, epigenomics, transcriptomics, proteomics, and metabolomics) for potentially powerful evolutionary insights. These in vitro techniques include ancestral protein resurrection, cell line experiments using primary, immortalized, and induced pluripotent stem cells, and CRISPR-Cas9 genetic manipulation. We also discuss how several of these methods can be used in vivo, for transgenic organism studies of human and nonhuman primate evolution. Throughout this review, we highlight example studies in which these approaches have already been used to inform our understanding of the evolutionary biology of modern and archaic humans and other primates while simultaneously identifying future opportunities for anthropologists to use this toolkit to help answer additional outstanding questions in evolutionary anthropology.
AB - In recent years, tools for functional genomic studies have become increasingly feasible for use by evolutionary anthropologists. In this review, we provide brief overviews of several exciting in vitro techniques that can be paired with “-omics” approaches (e.g., genomics, epigenomics, transcriptomics, proteomics, and metabolomics) for potentially powerful evolutionary insights. These in vitro techniques include ancestral protein resurrection, cell line experiments using primary, immortalized, and induced pluripotent stem cells, and CRISPR-Cas9 genetic manipulation. We also discuss how several of these methods can be used in vivo, for transgenic organism studies of human and nonhuman primate evolution. Throughout this review, we highlight example studies in which these approaches have already been used to inform our understanding of the evolutionary biology of modern and archaic humans and other primates while simultaneously identifying future opportunities for anthropologists to use this toolkit to help answer additional outstanding questions in evolutionary anthropology.
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U2 - 10.1002/evan.21825
DO - 10.1002/evan.21825
M3 - Review article
C2 - 32142200
AN - SCOPUS:85081572686
SN - 1060-1538
VL - 29
SP - 143
EP - 158
JO - Evolutionary anthropology
JF - Evolutionary anthropology
IS - 3
ER -