TY - JOUR
T1 - Subnuclear localization and phosphorylation of Epstein-Barr virus latent infection nuclear proteins
AU - Petti, Lisa
AU - Sample, Clare
AU - Kieff, Elliott
N1 - Funding Information:
David Liebowitz provided useful advice. This research was supported by Public Health Service Grant lR35CA47006-01 from the National Cancer Institute. Elliott Kieff is partially supported by grants from the Sandoz Company and from the Baxter Foundation. Clare Sample was supported by Fellowship Al07894 from the National Institute of Allergy and Infectious Diseases.
PY - 1990/6
Y1 - 1990/6
N2 - Functions of the six Epstein-Barr virus latent infection nuclear proteins (EBNA-1, -2, -3A, -3B, -3C, or-LP) in maintaining latent infection or cell growth transformation are only partially understood. Using antibodies specific for each EBNA in immunofluorescence microscopy, EBNA-2, -3A, and -3C localized to subnuclear granules which fill much of the nucleus, excluding nucleoli. EBNA-LP localized to a small number of discrete subnuclear particles, also excluding nucleoli. Only EBNA-1 associated with metaphase chromosomes. Concordantly, in biochemical nuclear fractionation studies, EBNA-1 was the major chromatin-associated EBNA. EBNA-1 also differed from the other EBNAs in the extent of its association with the nucleoplasm and in its lack of nuclear matrix association. EBNA-LP, -2, -3A, and -3C were associated with the nuclear matrix, although they were also found in the nucleoplasm and to a lesser extent in the chromatin fractions. Metabolic 32Pi-labeling of cells followed by two-dimensional gel electrophoresis showed that EBNA-LP could be resolved into multiple phosphorylated isoforms. EBNA-2 was also phosphorylated and many isoforms were detected by isoelectric focusing.
AB - Functions of the six Epstein-Barr virus latent infection nuclear proteins (EBNA-1, -2, -3A, -3B, -3C, or-LP) in maintaining latent infection or cell growth transformation are only partially understood. Using antibodies specific for each EBNA in immunofluorescence microscopy, EBNA-2, -3A, and -3C localized to subnuclear granules which fill much of the nucleus, excluding nucleoli. EBNA-LP localized to a small number of discrete subnuclear particles, also excluding nucleoli. Only EBNA-1 associated with metaphase chromosomes. Concordantly, in biochemical nuclear fractionation studies, EBNA-1 was the major chromatin-associated EBNA. EBNA-1 also differed from the other EBNAs in the extent of its association with the nucleoplasm and in its lack of nuclear matrix association. EBNA-LP, -2, -3A, and -3C were associated with the nuclear matrix, although they were also found in the nucleoplasm and to a lesser extent in the chromatin fractions. Metabolic 32Pi-labeling of cells followed by two-dimensional gel electrophoresis showed that EBNA-LP could be resolved into multiple phosphorylated isoforms. EBNA-2 was also phosphorylated and many isoforms were detected by isoelectric focusing.
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U2 - 10.1016/0042-6822(90)90027-O
DO - 10.1016/0042-6822(90)90027-O
M3 - Article
C2 - 2161150
AN - SCOPUS:0025370003
SN - 0042-6822
VL - 176
SP - 563
EP - 574
JO - Virology
JF - Virology
IS - 2
ER -