TY - JOUR
T1 - Subset-specific analysis of calcium fluxes in murine AIDS
AU - Moutschen, Michel
AU - Trebak, Mohamed
AU - Greimers, Roland
AU - Colombi, Sonia
AU - Boniver, Jacques
N1 - Funding Information:
This work is supported in part by the Fund for Medical Scientific Research (Belgium) (FRSM) and the Centre Anticancereux pres I'Universite de Liege (CAC). M. M. is a Senior Research Assistant of the National Fund for Scientific Research (Belgium) (FNRS). M. T. is supported by a research grant from the CAC. S. C. is a Research Fellow of the Institute for Industrial and Agronomic Scientific Research (IRSIA). We gratefully acknowledge Mrs E. Franzen-Detrooz and G. Zeimers for excellent technical assistance, and Dr Sandra Giannini for critical reading of the manuscript.
PY - 1996
Y1 - 1996
N2 - Infection of susceptible strains of mice with the Duplan strain of murine leukemia viruses induces a syndrome called MAIDS (murine acquired immunodeficiency syndrome) characterized by immunodeficiency and lymphoproliferation. In addition to a complete refractoriness of most subsets of lymphocytes to mitogen stimulation, the development of phenotypic abnormalities occurs such as the appearance of an abnormal CD4+ T cell subset lacking membrane Thy-1. This study was performed to compare the calcium responses during the early stages of MAIDS (week 9 or earlier) between T cells and B cells and between CD4+Thy-1- and CD4+Thy-1+ T cells. B cells were strikingly less affected than T cells: their baseline [Ca2+](i) did not significantly increase, and their calcium response to anti-IgM antibody and concanavalin A (Con A) was partially maintained. In contrast, the response to Con A was completely abolished in T cells. Interestingly, calcium mobilization in response to membrane receptor-independent stimuli such as ionophores and thapsigargin was strongly inhibited in T cells, while no such inhibition was found in B cells. In comparison with their CD4+Thy-1+ counterparts, CD4+Thy-1- T cells had blunted calcium responses in controls, as well as in infected mice. However, CD4+Thy-1+ T cells were also strikingly altered, suggesting that the loss of membrane Thy-1 could be associated with, but not directly responsible for abnormalities of calcium responses in CD4+ T cells from RadLV-Rs-infected mice.
AB - Infection of susceptible strains of mice with the Duplan strain of murine leukemia viruses induces a syndrome called MAIDS (murine acquired immunodeficiency syndrome) characterized by immunodeficiency and lymphoproliferation. In addition to a complete refractoriness of most subsets of lymphocytes to mitogen stimulation, the development of phenotypic abnormalities occurs such as the appearance of an abnormal CD4+ T cell subset lacking membrane Thy-1. This study was performed to compare the calcium responses during the early stages of MAIDS (week 9 or earlier) between T cells and B cells and between CD4+Thy-1- and CD4+Thy-1+ T cells. B cells were strikingly less affected than T cells: their baseline [Ca2+](i) did not significantly increase, and their calcium response to anti-IgM antibody and concanavalin A (Con A) was partially maintained. In contrast, the response to Con A was completely abolished in T cells. Interestingly, calcium mobilization in response to membrane receptor-independent stimuli such as ionophores and thapsigargin was strongly inhibited in T cells, while no such inhibition was found in B cells. In comparison with their CD4+Thy-1+ counterparts, CD4+Thy-1- T cells had blunted calcium responses in controls, as well as in infected mice. However, CD4+Thy-1+ T cells were also strikingly altered, suggesting that the loss of membrane Thy-1 could be associated with, but not directly responsible for abnormalities of calcium responses in CD4+ T cells from RadLV-Rs-infected mice.
UR - http://www.scopus.com/inward/record.url?scp=0029824765&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029824765&partnerID=8YFLogxK
U2 - 10.1093/intimm/8.11.1715
DO - 10.1093/intimm/8.11.1715
M3 - Article
C2 - 8943566
AN - SCOPUS:0029824765
SN - 0953-8178
VL - 8
SP - 1715
EP - 1727
JO - International immunology
JF - International immunology
IS - 11
ER -