Subtilisin catalysis of substrate anchored in cyclodextrin

K. Sandeep Prabhu; S. Candadai Ramadoss

doi:10.1007/BF02786022

Subtilisin catalysis of substrate anchored in cyclodextrin

K. Sandeep Prabhu, S. Candadai Ramadoss

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The N-benzoyl-L-tyrosine ethyl ester (BTEE) anchored in cyclodextrin (β and β-methyl CD) serves as an excellent substrate for subtilisin Carlsberg catalysis. The rate of hydrolysis was found to be approximately twofold higher than that of the methanolic substrate. The K(mapp) and V(max) values for the CD anchored substrates were significantly higher than the methanol- solubilized BTEE.

Original language	English (US)
Pages (from-to)	69-77
Number of pages	9
Journal	Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
Volume	69
Issue number	1
DOIs	https://doi.org/10.1007/BF02786022
State	Published - 1998

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology

Access to Document

10.1007/BF02786022

Cite this

@article{d471e370f8a94e16a20a25ea94006a8a,

title = "Subtilisin catalysis of substrate anchored in cyclodextrin",

abstract = "The N-benzoyl-L-tyrosine ethyl ester (BTEE) anchored in cyclodextrin (β and β-methyl CD) serves as an excellent substrate for subtilisin Carlsberg catalysis. The rate of hydrolysis was found to be approximately twofold higher than that of the methanolic substrate. The K(mapp) and V(max) values for the CD anchored substrates were significantly higher than the methanol- solubilized BTEE.",

author = "Prabhu, {K. Sandeep} and Ramadoss, {S. Candadai}",

note = "Funding Information: Division of Biological Sciences, Vittal Mallya Scientific Research Foundation, Post Box # 406, K. R. Road, Bangabre 560 004, India Funding Information: We thank Elmar Kolosa, Wacker-ChemieG mbH, Munich, Germany, for the generousg ift of ~-methyl-cyclodextrainn,d P. R. Krishnaswamy and P. V. SubbaR ao for their keen interesta nd encouragemenKt.. S. P. is gratefult o the Council of Scientifica nd Industrial Research( CSIR), New Delhi, for the award of SeniorR esearchF ellowship.",

year = "1998",

doi = "10.1007/BF02786022",

language = "English (US)",

volume = "69",

pages = "69--77",

journal = "Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology",

issn = "0273-2289",

publisher = "Springer",

number = "1",

}

TY - JOUR

T1 - Subtilisin catalysis of substrate anchored in cyclodextrin

AU - Prabhu, K. Sandeep

AU - Ramadoss, S. Candadai

N1 - Funding Information: Division of Biological Sciences, Vittal Mallya Scientific Research Foundation, Post Box # 406, K. R. Road, Bangabre 560 004, India Funding Information: We thank Elmar Kolosa, Wacker-ChemieG mbH, Munich, Germany, for the generousg ift of ~-methyl-cyclodextrainn,d P. R. Krishnaswamy and P. V. SubbaR ao for their keen interesta nd encouragemenKt.. S. P. is gratefult o the Council of Scientifica nd Industrial Research( CSIR), New Delhi, for the award of SeniorR esearchF ellowship.

PY - 1998

Y1 - 1998

N2 - The N-benzoyl-L-tyrosine ethyl ester (BTEE) anchored in cyclodextrin (β and β-methyl CD) serves as an excellent substrate for subtilisin Carlsberg catalysis. The rate of hydrolysis was found to be approximately twofold higher than that of the methanolic substrate. The K(mapp) and V(max) values for the CD anchored substrates were significantly higher than the methanol- solubilized BTEE.

AB - The N-benzoyl-L-tyrosine ethyl ester (BTEE) anchored in cyclodextrin (β and β-methyl CD) serves as an excellent substrate for subtilisin Carlsberg catalysis. The rate of hydrolysis was found to be approximately twofold higher than that of the methanolic substrate. The K(mapp) and V(max) values for the CD anchored substrates were significantly higher than the methanol- solubilized BTEE.

UR - http://www.scopus.com/inward/record.url?scp=0041097925&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0041097925&partnerID=8YFLogxK

U2 - 10.1007/BF02786022

DO - 10.1007/BF02786022

M3 - Article

AN - SCOPUS:0041097925

SN - 0273-2289

VL - 69

SP - 69

EP - 77

JO - Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology

JF - Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology

IS - 1

ER -

Subtilisin catalysis of substrate anchored in cyclodextrin

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this