TY - JOUR
T1 - Suppression of cell‐mediated lymphocytotoxicity against minor histocompatibility antigens mediated by Lyt‐1+Lyt−2+ T cells of stimulator‐strain origin
AU - Rammensee, Hans‐Georg ‐G
AU - Nagy, Zoltan A.
AU - Klein, Jan
PY - 1982
Y1 - 1982
N2 - Cultured, unprimed spleen cells suppress the generation of cytolytic T lymphocytes when added to mixed lymphocyte culture of cells disparate at minor histocompatibility (H) loci. Cells cultured for 2 to 3 days inhibit cell‐mediated lympholysis specifically in that they suppress only when they carry the same H antigen by which the stimulators activate the responding cells. However, culturing of cells for more than 3 days results also in the generation of nonspecific suppressor cells which inhibit the cell‐mediated lympholysis regardless whether they carry the stimulating antigens. The specific suppressor cells are T lymphocytes of the Lyt‐l+Lyt−2+ type. Fresh, uncultured spleen cells, although ineffective in vitro, can prevent the in vivo priming of minor H‐specific cytotoxic T lymphocyte precursors when injected i.v. into mice incompatible at minor H loci. This suppressive effect of the immunizing inoculum is abolished by irradiation (3300 rds) or by treatment with a monoclonal Thy‐1‐specific antibody and complement. Thus the suppressor T cell demonstrated here is of the “veto” cell type; that is, it probably suppresses minor H‐specific cells when the latter recognize the stimulating antigen on the surface of the suppressor cell itself.
AB - Cultured, unprimed spleen cells suppress the generation of cytolytic T lymphocytes when added to mixed lymphocyte culture of cells disparate at minor histocompatibility (H) loci. Cells cultured for 2 to 3 days inhibit cell‐mediated lympholysis specifically in that they suppress only when they carry the same H antigen by which the stimulators activate the responding cells. However, culturing of cells for more than 3 days results also in the generation of nonspecific suppressor cells which inhibit the cell‐mediated lympholysis regardless whether they carry the stimulating antigens. The specific suppressor cells are T lymphocytes of the Lyt‐l+Lyt−2+ type. Fresh, uncultured spleen cells, although ineffective in vitro, can prevent the in vivo priming of minor H‐specific cytotoxic T lymphocyte precursors when injected i.v. into mice incompatible at minor H loci. This suppressive effect of the immunizing inoculum is abolished by irradiation (3300 rds) or by treatment with a monoclonal Thy‐1‐specific antibody and complement. Thus the suppressor T cell demonstrated here is of the “veto” cell type; that is, it probably suppresses minor H‐specific cells when the latter recognize the stimulating antigen on the surface of the suppressor cell itself.
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U2 - 10.1002/eji.1830121107
DO - 10.1002/eji.1830121107
M3 - Article
C2 - 6217978
AN - SCOPUS:0020365220
SN - 0014-2980
VL - 12
SP - 930
EP - 934
JO - European Journal of Immunology
JF - European Journal of Immunology
IS - 11
ER -