TY - JOUR
T1 - Surface conformational and linear epitopes on HPV-16 and HPV-18 L1 virus-like particles as defined by monoclonal antibodies
AU - Christensen, Neil D.
AU - Dillner, Joakim
AU - Eklund, Carina
AU - Carter, Joseph J.
AU - Wipf, Gregory C.
AU - Reed, Cynthia A.
AU - Cladel, Nancy M.
AU - Galloway, Denise A.
N1 - Funding Information:
This study was supported by Public Health Service Grants CA56460 (N.D.C.), CA42792 (D.A.G.), and AI38382 (D.A.G.), a grant from the Cancer Research Foundation of America (N.D.C.) and the Jake Gittlen Memorial Golf Tournament. We thank Drs. Ben Jenson (Georgetown University, Washington, DC) and John Schiller (NIH, Bethesda, MD) for the generous gifts of the recombinant baculovirus expressing HPV-6 and BPV-1 L1, respectively, and Kristin Robinson for purification of the recombinant vaccinia viruses.
PY - 1996/9/1
Y1 - 1996/9/1
N2 - A panel of 24 monoclonal antibodies (MAbs) was generated against human papillomavirus (HPV) types 16 and 18 L1 virus-like particles (VLPs). The MAbs were screened for reactivity to a variety of VLPs prepared from HPV-6, -11, -16, -18, -31, -33, -35, and -45, cottontail rabbit papillomavirus, bovine papillomavirus type 1, and a set of 35 overlapping 20-amino-acid peptides spanning the entire HPV-16 L1 gene. Type-specific linear and conformational surface epitopes were detected as well as several cross-reactive linear epitopes that showed various levels of cross-reactivity between different genital HPV and animal papillomavirus L1s. Most of the linear epitopes were mapped using synthetic peptides, and the epitopes were identified as being either surface or buried within the VLP as defined by the pattern of reactivity in ELISA using intact and disrupted VLP antigen. These MAbs may be useful reagents to help define neutralizing epitopes of HPV-16 and -18 when infectivity assays become available, and to define the regions of L1 that are exposed on the surface or buried within the assembled capsid.
AB - A panel of 24 monoclonal antibodies (MAbs) was generated against human papillomavirus (HPV) types 16 and 18 L1 virus-like particles (VLPs). The MAbs were screened for reactivity to a variety of VLPs prepared from HPV-6, -11, -16, -18, -31, -33, -35, and -45, cottontail rabbit papillomavirus, bovine papillomavirus type 1, and a set of 35 overlapping 20-amino-acid peptides spanning the entire HPV-16 L1 gene. Type-specific linear and conformational surface epitopes were detected as well as several cross-reactive linear epitopes that showed various levels of cross-reactivity between different genital HPV and animal papillomavirus L1s. Most of the linear epitopes were mapped using synthetic peptides, and the epitopes were identified as being either surface or buried within the VLP as defined by the pattern of reactivity in ELISA using intact and disrupted VLP antigen. These MAbs may be useful reagents to help define neutralizing epitopes of HPV-16 and -18 when infectivity assays become available, and to define the regions of L1 that are exposed on the surface or buried within the assembled capsid.
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U2 - 10.1006/viro.1996.0466
DO - 10.1006/viro.1996.0466
M3 - Article
C2 - 8806551
AN - SCOPUS:0030245737
SN - 0042-6822
VL - 223
SP - 174
EP - 184
JO - Virology
JF - Virology
IS - 1
ER -