TY - JOUR
T1 - Surfactant protein C
T2 - Hormonal control of SP-C mRNA levels in vitro
AU - Veletza, S. V.
AU - Nichols, K. V.
AU - Gross, I.
AU - Lu, H.
AU - Dynia, D. W.
AU - Floros, J.
PY - 1992
Y1 - 1992
N2 - We have studied hormonal regulation of the surfactant protein C (SP-C) in fetal 18-day rat lung explants. SP-C mRNA was detected in Northern blots with a specific rat SP-C cDNA probe and quantified by densitometry. Treatment of the explants with dexamethasone resulted in a dose-dependent increase of the SP-C mRNA level. Transcriptional assays have shown that the regulation of SP- C mRNA by dexamethasone involves a transcriptional step. Administration of the cAMP analogues, 8-bromoadenosine 3',5'-cyclic monophosphate (8-BrcAMP) or dibutyryl adenosine 3',5'-cyclic monophosphate (DBcAMP), produced a dose- dependent increase of SP-C mRNA levels, with maximum stimulation observed at 200 μM. The thyroid hormone T3 had no effect on SP-C mRNA levels, whether administered alone or in combination with dexamethasone. Variation in the effects of the above hormones on three surfactant protein mRNAs, SP-A, SP-B and SP-C, indicates that the hormonal regulation of the surfactant proteins is a complex process and that each gene is, in part, differentially regulated.
AB - We have studied hormonal regulation of the surfactant protein C (SP-C) in fetal 18-day rat lung explants. SP-C mRNA was detected in Northern blots with a specific rat SP-C cDNA probe and quantified by densitometry. Treatment of the explants with dexamethasone resulted in a dose-dependent increase of the SP-C mRNA level. Transcriptional assays have shown that the regulation of SP- C mRNA by dexamethasone involves a transcriptional step. Administration of the cAMP analogues, 8-bromoadenosine 3',5'-cyclic monophosphate (8-BrcAMP) or dibutyryl adenosine 3',5'-cyclic monophosphate (DBcAMP), produced a dose- dependent increase of SP-C mRNA levels, with maximum stimulation observed at 200 μM. The thyroid hormone T3 had no effect on SP-C mRNA levels, whether administered alone or in combination with dexamethasone. Variation in the effects of the above hormones on three surfactant protein mRNAs, SP-A, SP-B and SP-C, indicates that the hormonal regulation of the surfactant proteins is a complex process and that each gene is, in part, differentially regulated.
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U2 - 10.1152/ajplung.1992.262.6.l684
DO - 10.1152/ajplung.1992.262.6.l684
M3 - Article
C2 - 1319687
AN - SCOPUS:0026659967
SN - 0002-9513
VL - 262
SP - L684-L687
JO - American Journal of Physiology - Lung Cellular and Molecular Physiology
JF - American Journal of Physiology - Lung Cellular and Molecular Physiology
IS - 6 6-6
ER -