TY - JOUR
T1 - Susceptibility of human neuroblastoma cell lines to cytotoxic T lymphocyte-mediated lysis
AU - Zier, Karen S.
AU - Pierson, Giuliana R.
AU - Brown, Valerie
PY - 1990/7
Y1 - 1990/7
N2 - The susceptibility of neuroblastoma cells to cytotoxic T lymphocyte (CTL)-mediated killing was investigated. Cytotoxic lines were generated by sensitizing peripheral blood lymphocytes against two stimulator cells, a neuroblastoma line, CHP-100, and normal allogeneic lymphocytes, LS. LS cells shared class I antigens with CHP-100, but in addition expressed class II antigens. The resulting cell lines strongly lysed both CHP-100 and LS cells, but poorly killed the natural killer (NK) target K562. Specific blocking of lysis by a monoclonal antibody directed against class I determinants and strong killing by the line following depletion of cells with NK or LAK markers demonstrated that this neuroblastoma line lysed by CTL.
AB - The susceptibility of neuroblastoma cells to cytotoxic T lymphocyte (CTL)-mediated killing was investigated. Cytotoxic lines were generated by sensitizing peripheral blood lymphocytes against two stimulator cells, a neuroblastoma line, CHP-100, and normal allogeneic lymphocytes, LS. LS cells shared class I antigens with CHP-100, but in addition expressed class II antigens. The resulting cell lines strongly lysed both CHP-100 and LS cells, but poorly killed the natural killer (NK) target K562. Specific blocking of lysis by a monoclonal antibody directed against class I determinants and strong killing by the line following depletion of cells with NK or LAK markers demonstrated that this neuroblastoma line lysed by CTL.
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U2 - 10.1016/0165-5728(90)90029-M
DO - 10.1016/0165-5728(90)90029-M
M3 - Article
C2 - 2362016
AN - SCOPUS:0025332775
SN - 0165-5728
VL - 28
SP - 153
EP - 160
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
IS - 2
ER -