Symbiotic mutants of rhizobium meliloti that uncouple plant from bacterial differentiation

Turlough M. Finan; Ann M. Hirsch; John A. Leigh; Eric Johansen; Gretchen A. Kuldau; Sarah Deegan; Graham C. Walker; Ethan R. Signer

doi:10.1016/0092-8674(85)90346-0

Symbiotic mutants of rhizobium meliloti that uncouple plant from bacterial differentiation

Turlough M. Finan
, Ann M. Hirsch
, John A. Leigh
, Eric Johansen
, Gretchen A. Kuldau
, Sarah Deegan
, Graham C. Walker
, Ethan R. Signer

Research output: Contribution to journal › Article › peer-review

184 Scopus citations

Abstract

Spontaneous mutants at a new symbiotic locus in Rhizobium meliloti SU47 are resistant to several phages and are conditionally insensitive to a monoclonal antibody to the bacterial surface, apparently because they are deficient in a wild-type exopolysaccharide. On alfalfa, the mutants do not curl root hairs, but penetrate the epidermis directly, forming nodules that contain no visible infection threads or "bacteroids" have a few bacteria in superficial intercellular spaces only and not within the nodule cells, and fail to fix nitrogen (Fix^-). Evidently, infection threads are not essential for cell proliferation and nodule formation, which are here induced by a bacterial signal at a distance and uncoupled from the bacterial differentiation that normally goes on as well.

Original language	English (US)
Pages (from-to)	869-877
Number of pages	9
Journal	Cell
Volume	40
Issue number	4
DOIs	https://doi.org/10.1016/0092-8674(85)90346-0
State	Published - Apr 1985

All Science Journal Classification (ASJC) codes

General Biochemistry, Genetics and Molecular Biology

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10.1016/0092-8674(85)90346-0

Cite this

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title = "Symbiotic mutants of rhizobium meliloti that uncouple plant from bacterial differentiation",

abstract = "Spontaneous mutants at a new symbiotic locus in Rhizobium meliloti SU47 are resistant to several phages and are conditionally insensitive to a monoclonal antibody to the bacterial surface, apparently because they are deficient in a wild-type exopolysaccharide. On alfalfa, the mutants do not curl root hairs, but penetrate the epidermis directly, forming nodules that contain no visible infection threads or {"}bacteroids{"} have a few bacteria in superficial intercellular spaces only and not within the nodule cells, and fail to fix nitrogen (Fix-). Evidently, infection threads are not essential for cell proliferation and nodule formation, which are here induced by a bacterial signal at a distance and uncoupled from the bacterial differentiation that normally goes on as well.",

author = "Finan, \{Turlough M.\} and Hirsch, \{Ann M.\} and Leigh, \{John A.\} and Eric Johansen and Kuldau, \{Gretchen A.\} and Sarah Deegan and Walker, \{Graham C.\} and Signer, \{Ethan R.\}",

note = "Funding Information: We thank Guido DeVos for Tn5-233, Karyn Moskowitz and Ed Seling for help with the scanning electron microscope, Kay Leland for the final photographs, Dietz Bauer and Harry Calvert for unpublished results, John Biglow for help in isolating mutants, and Agway, Inc. (Syracuse NY) for seeds of Medicago sativa L. var. Iroquois. This work was supported by grants from the U.S. Department of Agriculture (80CRCR-10498) and U.S. Department of Energy (DEDG0284ER13252) to E. R. S., from the U.S. Public Health Service(GM31030) to E. R. S. and G. C. W., from the National Science Foundation (PCM8316793) and the Research Corporation to A. M. H., and from the National Science Foundation (69A) to Wellesley College. J A. L. was supported by a Damon Runyon-Walter Winchell Cancer Fund Fellowship (DRG-641) E. J. was supported by a predoctoral fellowship from the National Chemicals and Distillers Corporation, and G. A. K. was supported by",

year = "1985",

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doi = "10.1016/0092-8674(85)90346-0",

language = "English (US)",

volume = "40",

pages = "869--877",

journal = "Cell",

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AU - Finan, Turlough M.

AU - Hirsch, Ann M.

AU - Leigh, John A.

AU - Johansen, Eric

AU - Kuldau, Gretchen A.

AU - Deegan, Sarah

AU - Walker, Graham C.

AU - Signer, Ethan R.

N1 - Funding Information: We thank Guido DeVos for Tn5-233, Karyn Moskowitz and Ed Seling for help with the scanning electron microscope, Kay Leland for the final photographs, Dietz Bauer and Harry Calvert for unpublished results, John Biglow for help in isolating mutants, and Agway, Inc. (Syracuse NY) for seeds of Medicago sativa L. var. Iroquois. This work was supported by grants from the U.S. Department of Agriculture (80CRCR-10498) and U.S. Department of Energy (DEDG0284ER13252) to E. R. S., from the U.S. Public Health Service(GM31030) to E. R. S. and G. C. W., from the National Science Foundation (PCM8316793) and the Research Corporation to A. M. H., and from the National Science Foundation (69A) to Wellesley College. J A. L. was supported by a Damon Runyon-Walter Winchell Cancer Fund Fellowship (DRG-641) E. J. was supported by a predoctoral fellowship from the National Chemicals and Distillers Corporation, and G. A. K. was supported by

PY - 1985/4

Y1 - 1985/4

N2 - Spontaneous mutants at a new symbiotic locus in Rhizobium meliloti SU47 are resistant to several phages and are conditionally insensitive to a monoclonal antibody to the bacterial surface, apparently because they are deficient in a wild-type exopolysaccharide. On alfalfa, the mutants do not curl root hairs, but penetrate the epidermis directly, forming nodules that contain no visible infection threads or "bacteroids" have a few bacteria in superficial intercellular spaces only and not within the nodule cells, and fail to fix nitrogen (Fix-). Evidently, infection threads are not essential for cell proliferation and nodule formation, which are here induced by a bacterial signal at a distance and uncoupled from the bacterial differentiation that normally goes on as well.

AB - Spontaneous mutants at a new symbiotic locus in Rhizobium meliloti SU47 are resistant to several phages and are conditionally insensitive to a monoclonal antibody to the bacterial surface, apparently because they are deficient in a wild-type exopolysaccharide. On alfalfa, the mutants do not curl root hairs, but penetrate the epidermis directly, forming nodules that contain no visible infection threads or "bacteroids" have a few bacteria in superficial intercellular spaces only and not within the nodule cells, and fail to fix nitrogen (Fix-). Evidently, infection threads are not essential for cell proliferation and nodule formation, which are here induced by a bacterial signal at a distance and uncoupled from the bacterial differentiation that normally goes on as well.

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Symbiotic mutants of rhizobium meliloti that uncouple plant from bacterial differentiation

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