TY - JOUR
T1 - Synergistic effect of rhbmp‐2 protein and nanotextured titanium alloy surface to improve osteogenic implant properties
AU - Mesa‐restrepo, Andrea
AU - Civantos, Ana
AU - Allain, Jean Paul
AU - Patiño, Edwin
AU - Alzate, Juan Fernando
AU - Balcázar, Norman
AU - Montes, Robinson
AU - Pavón, Juan José
AU - Rodríguez‐ortiz, José Antonio
AU - Torres, Yadir
N1 - Funding Information:
Funding: This work was supported by the Ministry of Science and Innovation of Spain under the grant PID2019‐109371GB‐I00, by the Junta de Andalucía–FEDER (Spain) through the Project Ref. US‐1259771, Colciencias (Departamento Administrativo de Ciencia, Tecnología e Innovación) un‐ der the grant COL‐13‐2‐16 and University of Antioquia Master′s Scholarship fund. The BMP‐2 production work was supported by AM LTDA and Transferencia Tecnológica from University of Antioquia under the grant CODI‐UdeA # 2017‐18192.
Funding Information:
This work was supported by the Ministry of Science and Innovation of Spain under the grant PID2019‐109371GB‐I00, by the Junta de Andalucía–FEDER (Spain) through the Project Ref. US‐1259771, Colciencias (Departamento Administrativo de Ciencia, Tecnología e Innovación) under the grant COL‐13‐2‐16 and University of Antioquia Master′s Scholarship fund. The BMP‐2 production work was supported by AM LTDA and Transferencia Tecnológica from University of Antioquia under the grant CODI‐UdeA # 2017‐18192. The authors would like to thank University of Antioquia and Colciencias for the funding, Sandra Arias and Camilo Jaramillo for the DIS treatment and Viviana Posada for tak-ing some of the SEM micrographs as well as professors Luz Marina Restrepo, Junes Villarraga and Gabriel Bedoya for their guidance. Surface characterization was performed in Frederick Seitz Materials Research Lab and all the in vitro experiments were conducted in Micro and Nanotechnol-ogy Lab in UIUC.
Publisher Copyright:
Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/3
Y1 - 2021/3
N2 - One of the major limitations during titanium (Ti) implant osseointegration is the poor cellular interactions at the biointerface. In the present study, the combined effect of recombinant human Bone Morphogenetic Protein‐2 (rhBMP‐2) and nanopatterned Ti6Al4V fabricated with Directed irradiation synthesis (DIS) is investigated in vitro. This environmentally‐friendly plasma uses ions to create self‐organized nanostructures on the surfaces. Nanocones (≈36.7 nm in DIS 80°) and thinner nanowalls (≈16.5 nm in DIS 60°) were fabricated depending on DIS incidence angle and observed via scanning electron microscopy. All samples have a similar crystalline structure and wettability, except for sandblasted/acid‐etched (SLA) and acid‐etched/anodized (Anodized) samples which are more hydrophilic. Biological results revealed that the viability and adhesion properties (vinculin expression and cell spreading) of DIS 80° with BMP‐2 were similar to those polished with BMP‐2, yet we observed more filopodia on DIS 80° (≈39 filopodia/cell) compared to the other samples (<30 filopodia/cell). BMP‐2 increased alkaline phosphatase activity in all samples, tending to be higher in DIS 80°. Moreover, in the mineralization studies, DIS 80° with BMP‐2 and Anodized with BMP‐2 increased the formation of calcium deposits (>3.3 fold) compared to polished with BMP‐ 2. Hence, this study shows there is a synergistic effect of BMP‐2 and DIS surface modification in improving Ti biological properties which could be applied to Ti bone implants to treat bone disease.
AB - One of the major limitations during titanium (Ti) implant osseointegration is the poor cellular interactions at the biointerface. In the present study, the combined effect of recombinant human Bone Morphogenetic Protein‐2 (rhBMP‐2) and nanopatterned Ti6Al4V fabricated with Directed irradiation synthesis (DIS) is investigated in vitro. This environmentally‐friendly plasma uses ions to create self‐organized nanostructures on the surfaces. Nanocones (≈36.7 nm in DIS 80°) and thinner nanowalls (≈16.5 nm in DIS 60°) were fabricated depending on DIS incidence angle and observed via scanning electron microscopy. All samples have a similar crystalline structure and wettability, except for sandblasted/acid‐etched (SLA) and acid‐etched/anodized (Anodized) samples which are more hydrophilic. Biological results revealed that the viability and adhesion properties (vinculin expression and cell spreading) of DIS 80° with BMP‐2 were similar to those polished with BMP‐2, yet we observed more filopodia on DIS 80° (≈39 filopodia/cell) compared to the other samples (<30 filopodia/cell). BMP‐2 increased alkaline phosphatase activity in all samples, tending to be higher in DIS 80°. Moreover, in the mineralization studies, DIS 80° with BMP‐2 and Anodized with BMP‐2 increased the formation of calcium deposits (>3.3 fold) compared to polished with BMP‐ 2. Hence, this study shows there is a synergistic effect of BMP‐2 and DIS surface modification in improving Ti biological properties which could be applied to Ti bone implants to treat bone disease.
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U2 - 10.3390/met11030464
DO - 10.3390/met11030464
M3 - Article
AN - SCOPUS:85102172609
SN - 2075-4701
VL - 11
SP - 1
EP - 20
JO - Metals
JF - Metals
IS - 3
M1 - 464
ER -
