Abstract
The protein product of the rodent neu oncogene, p185neu, is a tyrosine kinase with structural similarity to the epidermal growth factor receptor (EGFR). Transfection and subsequent overexpression of the human p185c-erbB-2 protein transforms NIH 3T3 cells in vitro. However, NIH 3T3 cells are not transformed by overexpressed rodent p185c-neu. NIH 3T3 transfectants overexpressing EGF receptors are not transformed unless EGF is added to the cultures, and, even then, they are incompletely transformed. Several groups have recently demonstrated EGF-induced, EGFR-mediated phosphorylation of p185c-neu. During efforts to characterize the interaction of p185c-neu with EGFR further, we created cell lines that simultaneously overexpress both p185c-neu and EGFR and observed that these cells become transformed. These observations demonstrate that two distinct, overexpressed tyrosine kinases can act synergistically to transform NIH 3T3 cells, thus identifying a novel mechanism that can lead to transformation.
Original language | English (US) |
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Pages (from-to) | 287-292 |
Number of pages | 6 |
Journal | Cell |
Volume | 58 |
Issue number | 2 |
DOIs | |
State | Published - Jul 28 1989 |
All Science Journal Classification (ASJC) codes
- General Biochemistry, Genetics and Molecular Biology