Synthesis and in vitro cancer cell targeting of folate-functionalized biodegradable amphiphilic dendrimer-like star polymers

Weiqiang Cao; Jing Zhou; Yong Wang; Lei Zhu

doi:10.1021/bm101154r

Synthesis and in vitro cancer cell targeting of folate-functionalized biodegradable amphiphilic dendrimer-like star polymers

Weiqiang Cao, Jing Zhou, Yong Wang, Lei Zhu

Research output: Contribution to journal › Article › peer-review

43 Scopus citations

Abstract

By coupling a well-defined PLLA star polymer with six carboxylic acid-terminated polyester dendrons based on 2,2-bis(hydroxymethyl)propionic acid, a biodegradable dendrimer-like star polymer (DLSP) with multiple carboxylic acid groups at the outer surface was successfully synthesized. Conjugation of amine-functionalized folic acids (FA) onto the DLSP yielded a folate-DLSP hybrid as a carrier for targeted drug delivery. The chemical structures were proven by proton nuclear magnetic resonance and size exclusion chromatography. The DLSPs could form unimolecular micelles with a mean particle size of about 18 nm, as determined by dynamic light scattering. Flow cytometry and confocal microscope studies revealed that the cellular uptake of the folate-DLSP hybrid against KB cells (overexpressed folate-receptor) was much higher than that of the neat DLSP (without FA) due to the folate receptor-mediated binding.

Original language	English (US)
Pages (from-to)	3680-3687
Number of pages	8
Journal	Biomacromolecules
Volume	11
Issue number	12
DOIs	https://doi.org/10.1021/bm101154r
State	Published - Dec 13 2010

All Science Journal Classification (ASJC) codes

Bioengineering
Biomaterials
Polymers and Plastics
Materials Chemistry

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1021/bm101154r

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title = "Synthesis and in vitro cancer cell targeting of folate-functionalized biodegradable amphiphilic dendrimer-like star polymers",

abstract = "By coupling a well-defined PLLA star polymer with six carboxylic acid-terminated polyester dendrons based on 2,2-bis(hydroxymethyl)propionic acid, a biodegradable dendrimer-like star polymer (DLSP) with multiple carboxylic acid groups at the outer surface was successfully synthesized. Conjugation of amine-functionalized folic acids (FA) onto the DLSP yielded a folate-DLSP hybrid as a carrier for targeted drug delivery. The chemical structures were proven by proton nuclear magnetic resonance and size exclusion chromatography. The DLSPs could form unimolecular micelles with a mean particle size of about 18 nm, as determined by dynamic light scattering. Flow cytometry and confocal microscope studies revealed that the cellular uptake of the folate-DLSP hybrid against KB cells (overexpressed folate-receptor) was much higher than that of the neat DLSP (without FA) due to the folate receptor-mediated binding.",

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T1 - Synthesis and in vitro cancer cell targeting of folate-functionalized biodegradable amphiphilic dendrimer-like star polymers

AU - Cao, Weiqiang

AU - Zhou, Jing

AU - Wang, Yong

AU - Zhu, Lei

PY - 2010/12/13

Y1 - 2010/12/13

N2 - By coupling a well-defined PLLA star polymer with six carboxylic acid-terminated polyester dendrons based on 2,2-bis(hydroxymethyl)propionic acid, a biodegradable dendrimer-like star polymer (DLSP) with multiple carboxylic acid groups at the outer surface was successfully synthesized. Conjugation of amine-functionalized folic acids (FA) onto the DLSP yielded a folate-DLSP hybrid as a carrier for targeted drug delivery. The chemical structures were proven by proton nuclear magnetic resonance and size exclusion chromatography. The DLSPs could form unimolecular micelles with a mean particle size of about 18 nm, as determined by dynamic light scattering. Flow cytometry and confocal microscope studies revealed that the cellular uptake of the folate-DLSP hybrid against KB cells (overexpressed folate-receptor) was much higher than that of the neat DLSP (without FA) due to the folate receptor-mediated binding.

AB - By coupling a well-defined PLLA star polymer with six carboxylic acid-terminated polyester dendrons based on 2,2-bis(hydroxymethyl)propionic acid, a biodegradable dendrimer-like star polymer (DLSP) with multiple carboxylic acid groups at the outer surface was successfully synthesized. Conjugation of amine-functionalized folic acids (FA) onto the DLSP yielded a folate-DLSP hybrid as a carrier for targeted drug delivery. The chemical structures were proven by proton nuclear magnetic resonance and size exclusion chromatography. The DLSPs could form unimolecular micelles with a mean particle size of about 18 nm, as determined by dynamic light scattering. Flow cytometry and confocal microscope studies revealed that the cellular uptake of the folate-DLSP hybrid against KB cells (overexpressed folate-receptor) was much higher than that of the neat DLSP (without FA) due to the folate receptor-mediated binding.

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Synthesis and in vitro cancer cell targeting of folate-functionalized biodegradable amphiphilic dendrimer-like star polymers

Abstract

All Science Journal Classification (ASJC) codes

UN SDGs

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