TY - JOUR
T1 - Synthesis of Chiral Carbo-Nanotweezers for Enantiospecific Recognition and DNA Duplex Winding in Cancer Cells
AU - Tripathi, Indu
AU - Misra, Santosh K.
AU - Ostadhossein, Fatemeh
AU - Srivastava, Indrajit
AU - Pan, Dipanjan
N1 - Funding Information:
*E-mail: [email protected]. ORCID Santosh K. Misra: 0000-0002-3313-4895 Indrajit Srivastava: 0000-0002-6864-0202 Dipanjan Pan: 0000-0003-0175-4704 Author Contributions The manuscript was written through contributions of all authors. All authors have given approval to the final version of the manuscript. Funding This project has been partially supported by University of Illinois, National Institute of Health (1R42HL135965−01A1 (D. Pan) and Children’s Discovery Institute for financial assistance. F.O. is supported by American heart association grant #18pre34080003/2018. Notes The authors declare the following competing financial interest(s): Professor Pan has three start-up companies; however, none of these entities supported this work.
Funding Information:
We acknowledge University of Illinois at Urbana−Champaign, National Institute of Health and Children’s Discovery Institute for financial assistance. TEM, zeta potential and XPS were performed at Frederick Seitz Materials Research Laboratory Central research facilities, UIUC. CD were acquired at Rogers Adam Laboratory, UIUC. Confocal studies were performed at the Beckman Institute.
Publisher Copyright:
© 2018 American Chemical Society.
PY - 2018/11/7
Y1 - 2018/11/7
N2 - Targeting the DNA of tumor cells with small molecules may offer effective clinical strategies for transcriptional inhibition. We unveil synthesis and characterization of ∼20 nm chiral carbon nanoparticles for enantiospecific recognition of DNA. Our approach inculcates chirality in carbon nanoparticles by controlled tethering of minor groove binders, i.e., Tröger's base (TB). The chiral particles positively enriched the cellular nucleus in MCF-7 breast cancer cells, irrespective of the TB asymmetry tethered on the particle surface, but negatively induced chiral carbon nanoparticles exhibited improved efficiency at inhibiting cell growth. Further studies indicated that these chiral particles act as nanotweezers to perturb the genomic DNA and induce apoptosis cascade in cancer cells.
AB - Targeting the DNA of tumor cells with small molecules may offer effective clinical strategies for transcriptional inhibition. We unveil synthesis and characterization of ∼20 nm chiral carbon nanoparticles for enantiospecific recognition of DNA. Our approach inculcates chirality in carbon nanoparticles by controlled tethering of minor groove binders, i.e., Tröger's base (TB). The chiral particles positively enriched the cellular nucleus in MCF-7 breast cancer cells, irrespective of the TB asymmetry tethered on the particle surface, but negatively induced chiral carbon nanoparticles exhibited improved efficiency at inhibiting cell growth. Further studies indicated that these chiral particles act as nanotweezers to perturb the genomic DNA and induce apoptosis cascade in cancer cells.
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U2 - 10.1021/acsami.8b15618
DO - 10.1021/acsami.8b15618
M3 - Article
C2 - 30300544
AN - SCOPUS:85055722480
SN - 1944-8244
VL - 10
SP - 37886
EP - 37897
JO - ACS Applied Materials and Interfaces
JF - ACS Applied Materials and Interfaces
IS - 44
ER -