TY - JOUR
T1 - Targeting hepatic cancer cells with pegylated dendrimers displaying N-Acetylgalactosamine and SP94 peptide ligands
AU - Medina, Scott H.
AU - Tiruchinapally, Gopinath
AU - Chevliakov, Maxim V.
AU - Durmaz, Yasemin Yuksel
AU - Stender, Rachell N.
AU - Ensminger, William D.
AU - Shewach, Donna S.
AU - Elsayed, Mohamed E.H.
PY - 2013/10
Y1 - 2013/10
N2 - Poly(amidoamine) (PAMAM) dendrimers are branched water-soluble polymers defined by consecutive generation numbers (Gn) indicating a parallel increase in size, molecular weight, and number of surface groups available for conjugation of bioactive agents. In this article, we compare the biodistribution of N-acetylgalactosamine (NAcGal)-targeted [14C]1-G5-(NH2)5-(Ac)108-(NAcGal)14 particles to non-targeted [14C]1-G5-(NH2)127 and PEGylated [14C]1-G5-(NH2)44-(Ac)73-(PEG)10 particles in a mouse hepatic cancer model. Results show that both NAcGal-targeted and non-targeted particles are rapidly cleared from the systemic circulation with high distribution to the liver. However, NAcGal-targeted particles exhibited 2.5-fold higher accumulation in tumor tissue compared to non-targeted ones. In comparison, PEGylated particles showed a 16-fold increase in plasma residence time and a 5-fold reduction in liver accumulation. These results motivated us to engineer new PEGylated G5 particles with PEG chains anchored to the G5 surface via acid-labile cis-aconityl linkages where the free PEG tips are functionalized with NAcGal or SP94 peptide to investigate their potential as targeting ligands for hepatic cancer cells as a function of sugar conformation (α versus β), ligand concentration (100-4000 nM), and incubation time (2 and 24 hours) compared to fluorescently (Fl)-labeled and non-targeted G5-(Fl)6-(NH2)122 and G5-(Fl)6-(Ac)107-(cPEG)15 particles. Results show G5-(Fl)6-(Ac)107-(cPEG[NAcGalβ])14 particles achieve faster uptake and higher intracellular concentrations in HepG2 cancer cells compared to other G5 particles while escaping the non-specific adsorption of serum protein and phagocytosis by Kupffer cells, which make these particles the ideal carrier for selective drug delivery into hepatic cancer cells.
AB - Poly(amidoamine) (PAMAM) dendrimers are branched water-soluble polymers defined by consecutive generation numbers (Gn) indicating a parallel increase in size, molecular weight, and number of surface groups available for conjugation of bioactive agents. In this article, we compare the biodistribution of N-acetylgalactosamine (NAcGal)-targeted [14C]1-G5-(NH2)5-(Ac)108-(NAcGal)14 particles to non-targeted [14C]1-G5-(NH2)127 and PEGylated [14C]1-G5-(NH2)44-(Ac)73-(PEG)10 particles in a mouse hepatic cancer model. Results show that both NAcGal-targeted and non-targeted particles are rapidly cleared from the systemic circulation with high distribution to the liver. However, NAcGal-targeted particles exhibited 2.5-fold higher accumulation in tumor tissue compared to non-targeted ones. In comparison, PEGylated particles showed a 16-fold increase in plasma residence time and a 5-fold reduction in liver accumulation. These results motivated us to engineer new PEGylated G5 particles with PEG chains anchored to the G5 surface via acid-labile cis-aconityl linkages where the free PEG tips are functionalized with NAcGal or SP94 peptide to investigate their potential as targeting ligands for hepatic cancer cells as a function of sugar conformation (α versus β), ligand concentration (100-4000 nM), and incubation time (2 and 24 hours) compared to fluorescently (Fl)-labeled and non-targeted G5-(Fl)6-(NH2)122 and G5-(Fl)6-(Ac)107-(cPEG)15 particles. Results show G5-(Fl)6-(Ac)107-(cPEG[NAcGalβ])14 particles achieve faster uptake and higher intracellular concentrations in HepG2 cancer cells compared to other G5 particles while escaping the non-specific adsorption of serum protein and phagocytosis by Kupffer cells, which make these particles the ideal carrier for selective drug delivery into hepatic cancer cells.
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U2 - 10.1002/adhm.201200406
DO - 10.1002/adhm.201200406
M3 - Article
C2 - 23554387
AN - SCOPUS:84877734275
SN - 2192-2640
VL - 2
SP - 1337
EP - 1350
JO - Advanced Healthcare Materials
JF - Advanced Healthcare Materials
IS - 10
ER -