TY - JOUR
T1 - Technical and statistical improvements for flow cytometric DNA analysis of paraffin‐embedded tissue
AU - Sickle‐Santanello, Brenda J.
AU - Farrar, William B.
AU - Decenzo, Jane F.
AU - Keyhani‐Rofagha, Sedigheh
AU - Klein, John
AU - Pearl, Dennis
AU - Laufman, Harry
AU - O'Toole, Robert V.
PY - 1988/11
Y1 - 1988/11
N2 - Flow cytometric DNA analysis of paraffinembedded solid tumors has permitted review of large series of archival tissue in attempts to relate abnormal DNA content to prognosis. Limitations of the technique include: (1) a laborious, time‐consuming procedure; (2) variation in technique between laboratories; and (3) lack of an objective method of computing DNA indices. Critical evaluaiton of our technique has shortened the time involved in dewaxing and rehydration, selectively utilized patient's own normal tissue as the internal standard, proved reproducibility of stored specimens, standardized DNA index computation, and developed a statistical analysis to confirm aneuploidy. These technical improvements and the development of a statistical analysis provide a way to shorten the procedure time and standardize the data generated from flow cytometric DNA analysis so as to improve the quality of retrospective reviews of paraffin‐embedded tumors and accelerate the definition of flow cytometry's role as a prognostic indicator.
AB - Flow cytometric DNA analysis of paraffinembedded solid tumors has permitted review of large series of archival tissue in attempts to relate abnormal DNA content to prognosis. Limitations of the technique include: (1) a laborious, time‐consuming procedure; (2) variation in technique between laboratories; and (3) lack of an objective method of computing DNA indices. Critical evaluaiton of our technique has shortened the time involved in dewaxing and rehydration, selectively utilized patient's own normal tissue as the internal standard, proved reproducibility of stored specimens, standardized DNA index computation, and developed a statistical analysis to confirm aneuploidy. These technical improvements and the development of a statistical analysis provide a way to shorten the procedure time and standardize the data generated from flow cytometric DNA analysis so as to improve the quality of retrospective reviews of paraffin‐embedded tumors and accelerate the definition of flow cytometry's role as a prognostic indicator.
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U2 - 10.1002/cyto.990090613
DO - 10.1002/cyto.990090613
M3 - Article
C2 - 3208624
AN - SCOPUS:0023756011
SN - 0196-4763
VL - 9
SP - 594
EP - 599
JO - Cytometry
JF - Cytometry
IS - 6
ER -