Technology dictates algorithms: recent developments in read alignment

Mohammed Alser, Jeremy Rotman, Dhrithi Deshpande, Kodi Taraszka, Huwenbo Shi, Pelin Icer Baykal, Harry Taegyun Yang, Victor Xue, Sergey Knyazev, Benjamin D. Singer, Brunilda Balliu, David Koslicki, Pavel Skums, Alex Zelikovsky, Can Alkan, Onur Mutlu, Serghei Mangul

    Research output: Contribution to journalReview articlepeer-review

    33 Scopus citations

    Abstract

    Aligning sequencing reads onto a reference is an essential step of the majority of genomic analysis pipelines. Computational algorithms for read alignment have evolved in accordance with technological advances, leading to today’s diverse array of alignment methods. We provide a systematic survey of algorithmic foundations and methodologies across 107 alignment methods, for both short and long reads. We provide a rigorous experimental evaluation of 11 read aligners to demonstrate the effect of these underlying algorithms on speed and efficiency of read alignment. We discuss how general alignment algorithms have been tailored to the specific needs of various domains in biology.

    Original languageEnglish (US)
    Article number249
    JournalGenome biology
    Volume22
    Issue number1
    DOIs
    StatePublished - Dec 2021

    All Science Journal Classification (ASJC) codes

    • Ecology, Evolution, Behavior and Systematics
    • Genetics
    • Cell Biology

    Fingerprint

    Dive into the research topics of 'Technology dictates algorithms: recent developments in read alignment'. Together they form a unique fingerprint.

    Cite this