Telomeric (TTAGGG)(n) sequences are associated with nucleolus organizer regions (NORs) in the wood lemming

Wan Sheng Liu; Karl Fredga

doi:10.1023/A:1009255517764

Telomeric (TTAGGG)(n) sequences are associated with nucleolus organizer regions (NORs) in the wood lemming

Wan Sheng Liu, Karl Fredga

Animal Science

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48 Scopus citations

Abstract

The distribution of the (TTAGGG)(n) telomeric sequence was studied in chromosomes of the wood lemming, Myopus schisticolor, by fluorescence in-situ hybridization. As expected, the hybridization signals were observed at telomeres of all chromosomes. However, quite a number of interstitial telomeric sites were present in the pericentric heterochromatic regions. Consistent strong hybridization signals were also seen at one terminus of chromosomes 5, 7 and 12-15. By post-hybridization G-banding and silver-staining, the large blocks of the telomeric sequences on chromosomes 5 and 12 were localized to nucleolus organizer regions (NORs).

Original language	English (US)
Pages (from-to)	235-240
Number of pages	6
Journal	Chromosome Research
Volume	7
Issue number	3
DOIs	https://doi.org/10.1023/A:1009255517764
State	Published - 1999

All Science Journal Classification (ASJC) codes

Genetics

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title = "Telomeric (TTAGGG)(n) sequences are associated with nucleolus organizer regions (NORs) in the wood lemming",

abstract = "The distribution of the (TTAGGG)(n) telomeric sequence was studied in chromosomes of the wood lemming, Myopus schisticolor, by fluorescence in-situ hybridization. As expected, the hybridization signals were observed at telomeres of all chromosomes. However, quite a number of interstitial telomeric sites were present in the pericentric heterochromatic regions. Consistent strong hybridization signals were also seen at one terminus of chromosomes 5, 7 and 12-15. By post-hybridization G-banding and silver-staining, the large blocks of the telomeric sequences on chromosomes 5 and 12 were localized to nucleolus organizer regions (NORs).",

author = "Liu, {Wan Sheng} and Karl Fredga",

note = "Funding Information: The authors thank Dr Bhanu P. Chowdhary for providing a biotin-labelled telomeric probe, and Professor Ingemar Gustavsson, Department of Animal Breeding and Genetics, SLU, Sweden, for supplying the digital image analysis system. This work was supported by grants from the Swedish Natural Science Research Council, the Nilsson–Ehle Fund and the Erik Philip–S{\"o}rensen's Foundation. The postdoctoral fellowship for W.-S.L. from the Swedish",

year = "1999",

doi = "10.1023/A:1009255517764",

language = "English (US)",

volume = "7",

pages = "235--240",

journal = "Chromosome Research",

issn = "0967-3849",

publisher = "Springer Netherlands",

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T1 - Telomeric (TTAGGG)(n) sequences are associated with nucleolus organizer regions (NORs) in the wood lemming

AU - Liu, Wan Sheng

AU - Fredga, Karl

N1 - Funding Information: The authors thank Dr Bhanu P. Chowdhary for providing a biotin-labelled telomeric probe, and Professor Ingemar Gustavsson, Department of Animal Breeding and Genetics, SLU, Sweden, for supplying the digital image analysis system. This work was supported by grants from the Swedish Natural Science Research Council, the Nilsson–Ehle Fund and the Erik Philip–Sörensen's Foundation. The postdoctoral fellowship for W.-S.L. from the Swedish

PY - 1999

Y1 - 1999

N2 - The distribution of the (TTAGGG)(n) telomeric sequence was studied in chromosomes of the wood lemming, Myopus schisticolor, by fluorescence in-situ hybridization. As expected, the hybridization signals were observed at telomeres of all chromosomes. However, quite a number of interstitial telomeric sites were present in the pericentric heterochromatic regions. Consistent strong hybridization signals were also seen at one terminus of chromosomes 5, 7 and 12-15. By post-hybridization G-banding and silver-staining, the large blocks of the telomeric sequences on chromosomes 5 and 12 were localized to nucleolus organizer regions (NORs).

AB - The distribution of the (TTAGGG)(n) telomeric sequence was studied in chromosomes of the wood lemming, Myopus schisticolor, by fluorescence in-situ hybridization. As expected, the hybridization signals were observed at telomeres of all chromosomes. However, quite a number of interstitial telomeric sites were present in the pericentric heterochromatic regions. Consistent strong hybridization signals were also seen at one terminus of chromosomes 5, 7 and 12-15. By post-hybridization G-banding and silver-staining, the large blocks of the telomeric sequences on chromosomes 5 and 12 were localized to nucleolus organizer regions (NORs).

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JO - Chromosome Research

JF - Chromosome Research

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ER -

Telomeric (TTAGGG)(n) sequences are associated with nucleolus organizer regions (NORs) in the wood lemming

Abstract

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