TY - JOUR
T1 - Temporal variation in genetic composition of migratory helicoverpa zea in peripheral populations
AU - Perera, Omaththage P.
AU - Fescemyer, Howard W.
AU - Fleischer, Shelby J.
AU - Abel, Craig A.
N1 - Funding Information:
Funding: Funding for work performed by HWF and SJF was from USDA-ARS Specific Cooperative Agreement 58-6402-5-066. This research was partially funded by interagency agreement 60-6066-6-002 with USDA Animal and Plant Health Inspection Service funded through Farm Bill.
Funding Information:
Funding for work performed by HWF and SJF was from USDA-ARS Specific Cooperative Agreement 58-6402-5-066. This research was partially funded by interagency agreement 60-6066-6-002 with USDA Animal and Plant Health Inspection Service funded through Farm Bill. Acknowledgments: We thank Calvin A Pierce, Priya Chatakondi, and Terry Jones (USDA ARS Southern Insect Management Research Unit, Stoneville, MS, USA) for genomic DNA extractions, Fanny Liu (USDA ARS Genomics and Bioinformatics Research Unit, Stoneville, MS, USA) for genotyping, Rhandi Ball for assistance with DAPC R package configuration, and Brad Coates, Crop Genetics and Corn Insects Research Unit, USDA-ARS, Ames, IA, for comments on earlier versions of the manuscript. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. USDA is an equal opportunity provider and employer.
Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2020/8
Y1 - 2020/8
N2 - Migrant populations of Helicoverpa zea (Boddie) captured during 2002, 2005, 2016, and 2018 from Landisville and Rock Springs in Pennsylvania, USA were genotyped using 85 single nucleotide polymorphism (SNP) markers. Samples (n = 702) genotyped were divided into 16 putative populations based on collection time and site. Fixation indices (F-statistics), analysis of molecular variance, and discriminant analysis of principal components were used to examine within and among population genetic variation. The observed and expected heterozygosity in putative populations ranged from 0.317–0.418 and 0.320–0.359, respectively. Broad range of FST (0.0–0.2742) and FIS (0.0–0.2330) values indicated different genotype frequencies between and within the populations, respectively. High genetic diversity within and low genetic differentiation between populations was found in 2002 and 2005. Interestingly, high genetic differentiation between populations from two collection sites observed in 2018 populations was not evident in within-site comparisons of putative populations collected on different dates during the season. The shift of H. zea population genetic makeup in 2018 may be influenced by multiple biotic and abiotic factors including tropical storms. Continued assessment of these peripheral populations of H. zea will be needed to assess the impacts of genetic changes on pest control and resistance management tactics.
AB - Migrant populations of Helicoverpa zea (Boddie) captured during 2002, 2005, 2016, and 2018 from Landisville and Rock Springs in Pennsylvania, USA were genotyped using 85 single nucleotide polymorphism (SNP) markers. Samples (n = 702) genotyped were divided into 16 putative populations based on collection time and site. Fixation indices (F-statistics), analysis of molecular variance, and discriminant analysis of principal components were used to examine within and among population genetic variation. The observed and expected heterozygosity in putative populations ranged from 0.317–0.418 and 0.320–0.359, respectively. Broad range of FST (0.0–0.2742) and FIS (0.0–0.2330) values indicated different genotype frequencies between and within the populations, respectively. High genetic diversity within and low genetic differentiation between populations was found in 2002 and 2005. Interestingly, high genetic differentiation between populations from two collection sites observed in 2018 populations was not evident in within-site comparisons of putative populations collected on different dates during the season. The shift of H. zea population genetic makeup in 2018 may be influenced by multiple biotic and abiotic factors including tropical storms. Continued assessment of these peripheral populations of H. zea will be needed to assess the impacts of genetic changes on pest control and resistance management tactics.
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U2 - 10.3390/insects11080463
DO - 10.3390/insects11080463
M3 - Article
C2 - 32717838
AN - SCOPUS:85088559667
SN - 2075-4450
VL - 11
SP - 1
EP - 20
JO - Insects
JF - Insects
IS - 8
M1 - 463
ER -