Tetranucleotide markers based on cosmids from human chromosome 13 library

G. M. Ivanova; D. A. Chistyakov; L. I. Fedorova; V. M. Zakhar'ev; E. Yu Kotova; V. M. Bannikov; A. S. Krasil'nikov; R. L. Turetskaya; K. I. Ratmanova; V. P. Veiko; A. V. Zelenin; N. K. Yankovsky; E. A. Braga

Tetranucleotide markers based on cosmids from human chromosome 13 library

G. M. Ivanova, D. A. Chistyakov, L. I. Fedorova, V. M. Zakhar'ev, E. Yu Kotova, V. M. Bannikov, A. S. Krasil'nikov, R. L. Turetskaya, K. I. Ratmanova, V. P. Veiko, A. V. Zelenin, N. K. Yankovsky, E. A. Braga

Research output: Contribution to journal › Article › peer-review

Abstract

New markers of the human chromosome 13 were generated using tetranucleotide microsatellites. The cosmids with GATG and GACT motifs isolated earlier from the chromosome 13 library [1] were analyzed with Southern blotting and located subregionally using fluorescence in situ hybridization. We have subcloned 16 cosmids located at the longer arm of the chromosome 13. Tetranucleotide repeats were detected in 14 sequences of microsatellites analyzed with standard and specific primers. Six markers were generated using PCR. The polymorphism of these loci was studied for groups of 30-140 unrelated individuals. Allele and genotype frequencies were analyzed for the three most informative markers (4-6 alleles, heterozigosity 0.64-0.75). The highly informative locus D13S699 appeared useful for practical paternity tests.

Original language	English (US)
Pages (from-to)	337-346
Number of pages	10
Journal	Molecular Biology
Volume	31
Issue number	3
State	Published - May 1997

All Science Journal Classification (ASJC) codes

Biophysics
Structural Biology

Cite this

@article{c3a1d11d0f4748d19574eab8705d8037,

title = "Tetranucleotide markers based on cosmids from human chromosome 13 library",

abstract = "New markers of the human chromosome 13 were generated using tetranucleotide microsatellites. The cosmids with GATG and GACT motifs isolated earlier from the chromosome 13 library [1] were analyzed with Southern blotting and located subregionally using fluorescence in situ hybridization. We have subcloned 16 cosmids located at the longer arm of the chromosome 13. Tetranucleotide repeats were detected in 14 sequences of microsatellites analyzed with standard and specific primers. Six markers were generated using PCR. The polymorphism of these loci was studied for groups of 30-140 unrelated individuals. Allele and genotype frequencies were analyzed for the three most informative markers (4-6 alleles, heterozigosity 0.64-0.75). The highly informative locus D13S699 appeared useful for practical paternity tests.",

author = "Ivanova, {G. M.} and Chistyakov, {D. A.} and Fedorova, {L. I.} and Zakhar'ev, {V. M.} and Kotova, {E. Yu} and Bannikov, {V. M.} and Krasil'nikov, {A. S.} and Turetskaya, {R. L.} and Ratmanova, {K. I.} and Veiko, {V. P.} and Zelenin, {A. V.} and Yankovsky, {N. K.} and Braga, {E. A.}",

year = "1997",

month = may,

language = "English (US)",

volume = "31",

pages = "337--346",

journal = "Molecular Biology",

issn = "0026-8933",

publisher = "Pleiades Publishing",

number = "3",

}

TY - JOUR

T1 - Tetranucleotide markers based on cosmids from human chromosome 13 library

AU - Ivanova, G. M.

AU - Chistyakov, D. A.

AU - Fedorova, L. I.

AU - Zakhar'ev, V. M.

AU - Kotova, E. Yu

AU - Bannikov, V. M.

AU - Krasil'nikov, A. S.

AU - Turetskaya, R. L.

AU - Ratmanova, K. I.

AU - Veiko, V. P.

AU - Zelenin, A. V.

AU - Yankovsky, N. K.

AU - Braga, E. A.

PY - 1997/5

Y1 - 1997/5

N2 - New markers of the human chromosome 13 were generated using tetranucleotide microsatellites. The cosmids with GATG and GACT motifs isolated earlier from the chromosome 13 library [1] were analyzed with Southern blotting and located subregionally using fluorescence in situ hybridization. We have subcloned 16 cosmids located at the longer arm of the chromosome 13. Tetranucleotide repeats were detected in 14 sequences of microsatellites analyzed with standard and specific primers. Six markers were generated using PCR. The polymorphism of these loci was studied for groups of 30-140 unrelated individuals. Allele and genotype frequencies were analyzed for the three most informative markers (4-6 alleles, heterozigosity 0.64-0.75). The highly informative locus D13S699 appeared useful for practical paternity tests.

AB - New markers of the human chromosome 13 were generated using tetranucleotide microsatellites. The cosmids with GATG and GACT motifs isolated earlier from the chromosome 13 library [1] were analyzed with Southern blotting and located subregionally using fluorescence in situ hybridization. We have subcloned 16 cosmids located at the longer arm of the chromosome 13. Tetranucleotide repeats were detected in 14 sequences of microsatellites analyzed with standard and specific primers. Six markers were generated using PCR. The polymorphism of these loci was studied for groups of 30-140 unrelated individuals. Allele and genotype frequencies were analyzed for the three most informative markers (4-6 alleles, heterozigosity 0.64-0.75). The highly informative locus D13S699 appeared useful for practical paternity tests.

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UR - http://www.scopus.com/inward/citedby.url?scp=0031495160&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0031495160

SN - 0026-8933

VL - 31

SP - 337

EP - 346

JO - Molecular Biology

JF - Molecular Biology

IS - 3

ER -

Tetranucleotide markers based on cosmids from human chromosome 13 library

Abstract

All Science Journal Classification (ASJC) codes

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