TGF-β-induced CD8⁺CD103⁺ regulatory T cells show potent therapeutic effect on chronic graft-versus-host disease lupus by suppressing B cells

Lupus nephritis is one of most severe complications of systemic erythematosus lupus and current approaches are not curative for lupus nephritis. Although CD4⁺Foxp3⁺ regulatory T cells (Treg) are crucial for prevention of autoimmunity, the therapeutic effect of these cells on lupus nephritis is not satisfactory. We previously reported that CD8⁺CD103⁺ Treg induced ex vivo with TGF-β1 and IL-2 (CD8⁺CD103⁺ iTreg), regardless of Foxp3 expression, displayed potent immunosuppressive effect on Th cell response and had therapeutic effect on Th cell-mediated colitis. Here, we tested whether CD8⁺CD103⁺ iTreg can ameliorate lupus nephritis and determined potential molecular mechanisms. Adoptive transfer of CD8⁺CD103⁺ iTreg but not control cells to chronic graft-versus-host disease with a typical lupus syndrome showed decreased levels of autoantibodies and proteinuria, reduced renal pathological lesions, lowered renal deposition of IgG/C3, and improved survival. CD8⁺CD103⁺ iTreg cells suppressed not only T helper cells but also B cell responses directly that may involve in both TGF-β and IL-10 signals. Using RNA-seq, we demonstrated CD8⁺CD103⁺ iTreg have its own unique expression profiles of transcription factors. Thus, current study has identified and extended the target cells of CD8⁺CD103⁺ iTreg and provided a possible application of this new iTreg subset on lupus nephritis and other autoimmune diseases.