TY - JOUR
T1 - The CDC25 Protein of Saccharomyces cerevisiae Promotes Exchange of Guanine Nucleotides Bound to Ras
AU - Jones, Sara
AU - Vignais, Marie Luce
AU - Broach, James R.
PY - 1991/5
Y1 - 1991/5
N2 - The product of the CDC25 gene of Saccharomyces cerevisiae, in its capacity as an activator of the RAS/cyclic AMP pathway, is required for initiation of the cell cycle. In this report, we provide an identification of Cdc25p, the product of the CDC25 gene, and evidence that it promotes exchange of guanine nucleotides bound to Ras in vitro. Extracts of strains containing high levels of Cdc25p catalyze both removal of GDP from and the concurrent binding of GTP to Ras. This same activity is also obtained with an immunopurified Cdc25p-β-galactosidase fusion protein, suggesting that Cdc25p participates directly in the exchange reaction. This biochemical activity is consistent with previous genetic analysis of CDC25 function.
AB - The product of the CDC25 gene of Saccharomyces cerevisiae, in its capacity as an activator of the RAS/cyclic AMP pathway, is required for initiation of the cell cycle. In this report, we provide an identification of Cdc25p, the product of the CDC25 gene, and evidence that it promotes exchange of guanine nucleotides bound to Ras in vitro. Extracts of strains containing high levels of Cdc25p catalyze both removal of GDP from and the concurrent binding of GTP to Ras. This same activity is also obtained with an immunopurified Cdc25p-β-galactosidase fusion protein, suggesting that Cdc25p participates directly in the exchange reaction. This biochemical activity is consistent with previous genetic analysis of CDC25 function.
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U2 - 10.1128/MCB.11.5.2641
DO - 10.1128/MCB.11.5.2641
M3 - Article
C2 - 2017169
AN - SCOPUS:0026315383
SN - 0270-7306
VL - 11
SP - 2641
EP - 2646
JO - Molecular and cellular biology
JF - Molecular and cellular biology
IS - 5
ER -