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The cleavage sites and localization of genes encoding the restriction endonucleases Eco1831I and Ecohi

  • Anatoly N. Kravetz
  • , Marina V. Zakharova
  • , Irina V. Beletskaya
  • , Elena V. Sineva
  • , Marat M. Denjmuchametov
  • , Sergey I. Petrov
  • , Larisa I. Glatman
  • , Alexander S. Solonin

Research output: Contribution to journalArticlepeer-review

Abstract

The restriction endonucleases Eco 1831I and Ecohi cleave before the first 5'-cytosine in the recognition sequence 5' -↓ CCSGG --3' 3' --GGSCC↑-5'(where S = G or C), generate 5-base 5' cohesive ends, and are encoded by homologous plasmids that are restricted in McrA+ hosts. Thus, they differ in their cleavage specificity from that of the BcnI isoschizomer, which cleaves after the second 5' cytosine.

Original languageEnglish (US)
Pages (from-to)153-154
Number of pages2
JournalGene
Volume129
Issue number1
DOIs
StatePublished - Jul 15 1993

All Science Journal Classification (ASJC) codes

  • Genetics

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