TY - JOUR
T1 - The Drosophila zfh-1 and zfh-2 genes encode novel proteins containing both zinc-finger and homeodomain motifs
AU - Fortini, Mark E.
AU - Lai, Zhichun
AU - Rubin, Gerald M.
N1 - Funding Information:
We would like to thank Todd Laverty for performing the polytene chromosome in situ hybridizations, Greg Harris, Dean Smith, and Charles Zuker for collaborating on the construction of the Agtll retinal cDNA expression library, Kai Zinn and Corey Goodman for providing the Agtll embryonic cDNA library, and Kevin Moses for providing the AFIX genomic library. The manuscript was improved by the comments of Michael Bate, Iswar Hariharan, Steven Harrison, and Marek Mlodzik. This work was supported by NIH Grant GM33135 to G.M.R.
PY - 1991/6
Y1 - 1991/6
N2 - Two of the most common DNA-binding motifs found in eukaryotic transcriptional regulatory proteins are the homeodomain and the C2H2 zinc finger. In Drosophila, homeodomain and zinc-finger proteins have been implicated in a wide variety of developmental processes. Until now, no proteins have been described in which both these DNA-binding motifs are present. We report here the isolation of genes encoding two such Drosophila proteins from a cDNA expression library. The product of the zfh-1 gene (zinc-finger homeodomain protein 1) contains one homeodomain and nine C2H2 zinc fingers. The product of the zfh-2 gene processes three homeodomains and sixteen C2H2 zinc fingers. For zfh-1, antisera raised against nonoverlapping regions of the gene product all recognize a 145 kDa protein on protein immunoblots, suggesting that the different DNA-binding motifs are actually all present in the mature gene product. The novel arrangement of interspersed homeodomain and zinc-finger motifs in the primary sequences of the zfh-1 and zfh-2 gene products may signify an unusual mechanism of transcriptional regulation by these proteins.
AB - Two of the most common DNA-binding motifs found in eukaryotic transcriptional regulatory proteins are the homeodomain and the C2H2 zinc finger. In Drosophila, homeodomain and zinc-finger proteins have been implicated in a wide variety of developmental processes. Until now, no proteins have been described in which both these DNA-binding motifs are present. We report here the isolation of genes encoding two such Drosophila proteins from a cDNA expression library. The product of the zfh-1 gene (zinc-finger homeodomain protein 1) contains one homeodomain and nine C2H2 zinc fingers. The product of the zfh-2 gene processes three homeodomains and sixteen C2H2 zinc fingers. For zfh-1, antisera raised against nonoverlapping regions of the gene product all recognize a 145 kDa protein on protein immunoblots, suggesting that the different DNA-binding motifs are actually all present in the mature gene product. The novel arrangement of interspersed homeodomain and zinc-finger motifs in the primary sequences of the zfh-1 and zfh-2 gene products may signify an unusual mechanism of transcriptional regulation by these proteins.
UR - https://www.scopus.com/pages/publications/0025812355
UR - https://www.scopus.com/pages/publications/0025812355#tab=citedBy
U2 - 10.1016/0925-4773(91)90048-B
DO - 10.1016/0925-4773(91)90048-B
M3 - Article
C2 - 1680376
AN - SCOPUS:0025812355
SN - 0925-4773
VL - 34
SP - 113
EP - 122
JO - Mechanisms of Development
JF - Mechanisms of Development
IS - 2-3
ER -