The effect of hydralazine on arachidonic acid metabolism in isolated, washed human platelets

J. R. Luderer; L. M. Demers; R. W. Janson; C. T. Nomides; A. H. Hayes

The effect of hydralazine on arachidonic acid metabolism in isolated, washed human platelets

J. R. Luderer, L. M. Demers, R. W. Janson, C. T. Nomides, A. H. Hayes

Department of Pathology and Laboratory Medicine

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

The effects of hydralazine on arachidonic acid metabolism were studied in isolated washed human platelets. The washed platelets (10 ⁹/ml) were exposed to ¹⁴C-arachidonic acid and incubated for 15 minutes in a platelet aggregometer (37°C). The media was then extracted and the reaction products separated by means of reversed phase high pressure liquid chromatography. In this system, hydralazine inhibited the production of TXB ₂ (the stable metabolite of TXA ₂) and enhanced the production of PGF ₂(α) and PGE ₂ in a dose dependent fashion. Indomethacin, blocked the production of all prostaglandin compounds normally synthesized by platelets. These results suggest that hydralazine is a specific thromboxane synthetase inhibitor and that its vasodilatory effects might be related to its ability to block the production of an endogenous vasoconstrictor (i.e. TXA ₂).

Original language	English (US)
Pages (from-to)	43-52
Number of pages	10
Journal	Research Communications in Chemical Pathology and Pharmacology
Volume	28
Issue number	1
State	Published - Jun 20 1980

All Science Journal Classification (ASJC) codes

Pathology and Forensic Medicine
Toxicology
Pharmacology
Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

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abstract = "The effects of hydralazine on arachidonic acid metabolism were studied in isolated washed human platelets. The washed platelets (10 9/ml) were exposed to 14C-arachidonic acid and incubated for 15 minutes in a platelet aggregometer (37°C). The media was then extracted and the reaction products separated by means of reversed phase high pressure liquid chromatography. In this system, hydralazine inhibited the production of TXB 2 (the stable metabolite of TXA 2) and enhanced the production of PGF 2(α) and PGE 2 in a dose dependent fashion. Indomethacin, blocked the production of all prostaglandin compounds normally synthesized by platelets. These results suggest that hydralazine is a specific thromboxane synthetase inhibitor and that its vasodilatory effects might be related to its ability to block the production of an endogenous vasoconstrictor (i.e. TXA 2).",

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AU - Luderer, J. R.

AU - Demers, L. M.

AU - Janson, R. W.

AU - Nomides, C. T.

AU - Hayes, A. H.

PY - 1980/6/20

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N2 - The effects of hydralazine on arachidonic acid metabolism were studied in isolated washed human platelets. The washed platelets (10 9/ml) were exposed to 14C-arachidonic acid and incubated for 15 minutes in a platelet aggregometer (37°C). The media was then extracted and the reaction products separated by means of reversed phase high pressure liquid chromatography. In this system, hydralazine inhibited the production of TXB 2 (the stable metabolite of TXA 2) and enhanced the production of PGF 2(α) and PGE 2 in a dose dependent fashion. Indomethacin, blocked the production of all prostaglandin compounds normally synthesized by platelets. These results suggest that hydralazine is a specific thromboxane synthetase inhibitor and that its vasodilatory effects might be related to its ability to block the production of an endogenous vasoconstrictor (i.e. TXA 2).

AB - The effects of hydralazine on arachidonic acid metabolism were studied in isolated washed human platelets. The washed platelets (10 9/ml) were exposed to 14C-arachidonic acid and incubated for 15 minutes in a platelet aggregometer (37°C). The media was then extracted and the reaction products separated by means of reversed phase high pressure liquid chromatography. In this system, hydralazine inhibited the production of TXB 2 (the stable metabolite of TXA 2) and enhanced the production of PGF 2(α) and PGE 2 in a dose dependent fashion. Indomethacin, blocked the production of all prostaglandin compounds normally synthesized by platelets. These results suggest that hydralazine is a specific thromboxane synthetase inhibitor and that its vasodilatory effects might be related to its ability to block the production of an endogenous vasoconstrictor (i.e. TXA 2).

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The effect of hydralazine on arachidonic acid metabolism in isolated, washed human platelets

Abstract

All Science Journal Classification (ASJC) codes

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