TY - JOUR
T1 - The effect of rumen digesta inoculation on the time course of recovery from classical diet-induced milk fat depression in dairy cows
AU - Rico, D. E.
AU - Ying, Y.
AU - Clarke, A. R.
AU - Harvatine, K. J.
N1 - Funding Information:
The authors gratefully acknowledge the technical assistance of K. Proschold, E. Waumbaugh, and L. W. Rottman-Gredell (Penn State University, University Park PA). Gratitude is also expressed to the Pennsylvania State University Dairy Cattle Research and Education Center and its staff. This project was partially supported by the College of Agriculture Graduate Student Competitive Grant Program at Penn State granted to D. E. Rico and Agriculture and Food Research Initiative Competitive Grant no. 2010-65206-20723 from the USDA National Institute of Food and Agriculture (Washington, DC) . Soybean oil was graciously donated by Wenger's Feed Mill Inc. (Rheems, PA) .
PY - 2014/6
Y1 - 2014/6
N2 - Ten ruminally cannulated cows were used in a crossover design that investigated the effect of rumen digesta inoculation from non-milk fat-depressed cows on recovery from classical diet-induced milk fat depression (MFD) characterized by reduced fat yield, reduced de novo milk fat synthesis, and increased alternate trans isomers. Two additional cows fed a high-fiber and low-polyunsaturated fatty acid (FA) diet (31.8% neutral detergent fiber, 4.2% FA, and 1.2% C18:2) were used as rumen digesta donors. Milk fat depression was induced during the first 10. d of each period by feeding a low-fiber and high-polyunsaturated FA diet (induction; 26.1% neutral detergent fiber, 5.8% FA, and 1.9% C18:2), resulting in a 30% decrease in milk fat yield. A recovery phase followed where all cows were switched to the high-forage, low-polyunsaturated FA diet and were allocated to (1) control (no inoculation) or (2) ruminal inoculation with donor cow digesta (8. kg/d for 6. d). Milk yield and composition were measured every 3. d. Milk yield progressively decreased during recovery. Milk fat concentration increased progressively during the recovery phase and no effect of treatment existed at any time point. Also, no treatment effect of milk fat yield was detected. The concentration of milk de novo FA increased progressively during recovery for both treatments and was higher for inoculated compared with control cows on d 6. In agreement, milk fat concentration of trans-10,. cis-12 conjugated linoleic acid decreased progressively in both treatments and was lower in inoculated cows on d 3 and 6. Ruminal inoculation from non-milk fat-depressed cows did not change milk fat yield, but slightly accelerated the rate of recovery of de novo FA synthesis and normal ruminal FA biohydrogenation, demonstrating a possible opportunity for other interventions that improve the ruminal environment to accelerate recovery from this condition.
AB - Ten ruminally cannulated cows were used in a crossover design that investigated the effect of rumen digesta inoculation from non-milk fat-depressed cows on recovery from classical diet-induced milk fat depression (MFD) characterized by reduced fat yield, reduced de novo milk fat synthesis, and increased alternate trans isomers. Two additional cows fed a high-fiber and low-polyunsaturated fatty acid (FA) diet (31.8% neutral detergent fiber, 4.2% FA, and 1.2% C18:2) were used as rumen digesta donors. Milk fat depression was induced during the first 10. d of each period by feeding a low-fiber and high-polyunsaturated FA diet (induction; 26.1% neutral detergent fiber, 5.8% FA, and 1.9% C18:2), resulting in a 30% decrease in milk fat yield. A recovery phase followed where all cows were switched to the high-forage, low-polyunsaturated FA diet and were allocated to (1) control (no inoculation) or (2) ruminal inoculation with donor cow digesta (8. kg/d for 6. d). Milk yield and composition were measured every 3. d. Milk yield progressively decreased during recovery. Milk fat concentration increased progressively during the recovery phase and no effect of treatment existed at any time point. Also, no treatment effect of milk fat yield was detected. The concentration of milk de novo FA increased progressively during recovery for both treatments and was higher for inoculated compared with control cows on d 6. In agreement, milk fat concentration of trans-10,. cis-12 conjugated linoleic acid decreased progressively in both treatments and was lower in inoculated cows on d 3 and 6. Ruminal inoculation from non-milk fat-depressed cows did not change milk fat yield, but slightly accelerated the rate of recovery of de novo FA synthesis and normal ruminal FA biohydrogenation, demonstrating a possible opportunity for other interventions that improve the ruminal environment to accelerate recovery from this condition.
UR - http://www.scopus.com/inward/record.url?scp=84901005518&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84901005518&partnerID=8YFLogxK
U2 - 10.3168/jds.2013-7342
DO - 10.3168/jds.2013-7342
M3 - Article
C2 - 24746126
AN - SCOPUS:84901005518
SN - 0022-0302
VL - 97
SP - 3752
EP - 3760
JO - Journal of dairy science
JF - Journal of dairy science
IS - 6
ER -