TY - JOUR
T1 - The effect of selenium enrichment on baker's yeast proteome
AU - El-Bayoumy, Karam
AU - Das, Arunangshu
AU - Russell, Stephen
AU - Wolfe, Steven
AU - Jordan, Rick
AU - Renganathan, Kutralanathan
AU - Loughran, Thomas P.
AU - Somiari, Richard
N1 - Funding Information:
This work was supported in part by seed funds from the Penn State Hershey Cancer Institute and by the NCI Grant R01 CA127729 . We thank Dr. Judith Bond for her constructive comments and Cypress Company for providing both SEY and RY.
PY - 2012/1/4
Y1 - 2012/1/4
N2 - The use of regular yeast (RY) and selenium-enriched yeast (SEY) as dietary supplement is of interest because the Nutritional Prevention of Cancer (NPC) trial revealed that SEY but not RY decreased the incidence of prostate cancer (PC). Using two-dimensional difference in gel electrophoresis (2D-DIGE)-tandem mass spectrometry (MS/MS) approach, we performed proteomic analysis of RY and SEY to identify proteins that are differentially expressed as a result of selenium enrichment. 2D-DIGE revealed 96 candidate protein spots that were differentially expressed (p ≤ 0.05) between SEY and RY. The 96 spots were selected, sequenced by LC/MS/MS and 37 proteins were unequivocally identified. The 37 identified proteins were verified with ProteinProphet software and mapped to existing Gene Ontology categories. Furthermore, the expression profile of 5 of the proteins with validated or putative roles in the carcinogenesis process, and for which antibodies against human forms of the proteins are available commercially was verified by western analysis. This study provides evidence for the first time that SEY contains higher levels of Pyruvate Kinase, HSP70, and Elongation factor 2 and lower levels of Eukaryotic Translation Initiation Factor 5A-2 and Triosephosphate Isomerase than those found in RY.
AB - The use of regular yeast (RY) and selenium-enriched yeast (SEY) as dietary supplement is of interest because the Nutritional Prevention of Cancer (NPC) trial revealed that SEY but not RY decreased the incidence of prostate cancer (PC). Using two-dimensional difference in gel electrophoresis (2D-DIGE)-tandem mass spectrometry (MS/MS) approach, we performed proteomic analysis of RY and SEY to identify proteins that are differentially expressed as a result of selenium enrichment. 2D-DIGE revealed 96 candidate protein spots that were differentially expressed (p ≤ 0.05) between SEY and RY. The 96 spots were selected, sequenced by LC/MS/MS and 37 proteins were unequivocally identified. The 37 identified proteins were verified with ProteinProphet software and mapped to existing Gene Ontology categories. Furthermore, the expression profile of 5 of the proteins with validated or putative roles in the carcinogenesis process, and for which antibodies against human forms of the proteins are available commercially was verified by western analysis. This study provides evidence for the first time that SEY contains higher levels of Pyruvate Kinase, HSP70, and Elongation factor 2 and lower levels of Eukaryotic Translation Initiation Factor 5A-2 and Triosephosphate Isomerase than those found in RY.
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U2 - 10.1016/j.jprot.2011.10.013
DO - 10.1016/j.jprot.2011.10.013
M3 - Article
C2 - 22067702
AN - SCOPUS:84355166431
SN - 1874-3919
VL - 75
SP - 1018
EP - 1030
JO - Journal of Proteomics
JF - Journal of Proteomics
IS - 3
ER -
