The effects of α-lactalbumin and glycomacropeptide on the association of CaCo-2 cells by enteropathogenic Escherichia coli, Salmonella typhimuriumand Shigella flexneri

Wolfram M. Brück; Shannon L. Kelleher; Glenn R. Gibson; Gitte Graverholt; Bo L. Lönnerdal

doi:10.1111/j.1574-6968.2006.00268.x

The effects of α-lactalbumin and glycomacropeptide on the association of CaCo-2 cells by enteropathogenic Escherichia coli, Salmonella typhimuriumand Shigella flexneri

Wolfram M. Brück, Shannon L. Kelleher, Glenn R. Gibson, Gitte Graverholt, Bo L. Lönnerdal

Research output: Contribution to journal › Article › peer-review

52 Scopus citations

Abstract

Two milk components, α-lactalbumin (α-La) and glycomacropeptide (GMP) may inhibit intestinal infection/intoxification. ³[H] thymidine-labeled enteropathogenic Escherichia coli (EPEC), Salmonella typhimurium (ATCC 6994) or Shigella flexneri (ATCC 9199) were introduced to CaCo-2 cultures and their association with CaCo-2 cells was assessed. Undigested, pepsin-digested and pepsin- and pancreatin-digested α-lactalbumin and glycomacropeptide inhibited association. Thus, milk supplemented with α-lactalbumin and glycomacropeptide might be effective in inhibiting associations of the pathogens EPEC, Salmonella typhimurium, and Shigella flexneri to intestinal cells.

Original language	English (US)
Pages (from-to)	158-162
Number of pages	5
Journal	FEMS Microbiology Letters
Volume	259
Issue number	1
DOIs	https://doi.org/10.1111/j.1574-6968.2006.00268.x
State	Published - Jun 2006

All Science Journal Classification (ASJC) codes

Microbiology
Molecular Biology
Genetics

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10.1111/j.1574-6968.2006.00268.x

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title = "The effects of α-lactalbumin and glycomacropeptide on the association of CaCo-2 cells by enteropathogenic Escherichia coli, Salmonella typhimuriumand Shigella flexneri",

abstract = "Two milk components, α-lactalbumin (α-La) and glycomacropeptide (GMP) may inhibit intestinal infection/intoxification. 3[H] thymidine-labeled enteropathogenic Escherichia coli (EPEC), Salmonella typhimurium (ATCC 6994) or Shigella flexneri (ATCC 9199) were introduced to CaCo-2 cultures and their association with CaCo-2 cells was assessed. Undigested, pepsin-digested and pepsin- and pancreatin-digested α-lactalbumin and glycomacropeptide inhibited association. Thus, milk supplemented with α-lactalbumin and glycomacropeptide might be effective in inhibiting associations of the pathogens EPEC, Salmonella typhimurium, and Shigella flexneri to intestinal cells.",

author = "Br{\"u}ck, {Wolfram M.} and Kelleher, {Shannon L.} and Gibson, {Glenn R.} and Gitte Graverholt and L{\"o}nnerdal, {Bo L.}",

year = "2006",

month = jun,

doi = "10.1111/j.1574-6968.2006.00268.x",

language = "English (US)",

volume = "259",

pages = "158--162",

journal = "FEMS Microbiology Letters",

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publisher = "Oxford University Press",

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}

The effects of α-lactalbumin and glycomacropeptide on the association of CaCo-2 cells by enteropathogenic Escherichia coli, Salmonella typhimuriumand Shigella flexneri. / Brück, Wolfram M.; Kelleher, Shannon L.; Gibson, Glenn R. et al.
In: FEMS Microbiology Letters, Vol. 259, No. 1, 06.2006, p. 158-162.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - The effects of α-lactalbumin and glycomacropeptide on the association of CaCo-2 cells by enteropathogenic Escherichia coli, Salmonella typhimuriumand Shigella flexneri

AU - Brück, Wolfram M.

AU - Kelleher, Shannon L.

AU - Gibson, Glenn R.

AU - Graverholt, Gitte

AU - Lönnerdal, Bo L.

PY - 2006/6

Y1 - 2006/6

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AB - Two milk components, α-lactalbumin (α-La) and glycomacropeptide (GMP) may inhibit intestinal infection/intoxification. 3[H] thymidine-labeled enteropathogenic Escherichia coli (EPEC), Salmonella typhimurium (ATCC 6994) or Shigella flexneri (ATCC 9199) were introduced to CaCo-2 cultures and their association with CaCo-2 cells was assessed. Undigested, pepsin-digested and pepsin- and pancreatin-digested α-lactalbumin and glycomacropeptide inhibited association. Thus, milk supplemented with α-lactalbumin and glycomacropeptide might be effective in inhibiting associations of the pathogens EPEC, Salmonella typhimurium, and Shigella flexneri to intestinal cells.

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The effects of α-lactalbumin and glycomacropeptide on the association of CaCo-2 cells by enteropathogenic Escherichia coli, Salmonella typhimuriumand Shigella flexneri

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