TY - JOUR
T1 - The effects of age on rabbit MCL fibroblast matrix synthesis in response to TGF-β1 or EGF
AU - Deie, Masataka
AU - Marui, Takashi
AU - Allen, Christina R.
AU - Hildebrand, Kevin A.
AU - Georgescu, Helga I.
AU - Niyibizi, Christopher
AU - Woo, Savio L.Y.
N1 - Funding Information:
This investigation was supported by NIH AR 41820. We thank Dr Christopher H. Evans for his technical advice and David Smith, Karl Kavalkovich and Rebecca Levine for their technical assistance.
PY - 1997/8
Y1 - 1997/8
N2 - In this study, we examined the effects of age on collagen and total protein synthesis by ligament fibroblasts in response to growth factors. Three different doses of transforming growth factor-β1 (TGF-β1) or epidermal growth factor (EGF) were individually added to in vitro fibroblast cultures from the medial collateral ligament (MCL) of skeletally immature (age 3 months), mature (age 12 months) and senescent (age 48-51 months) rabbits. Analysis of the effects of age revealed that fibroblasts from senescent rabbits produced significantly less collagen in response to TGF-β1 or EGF stimulation when compared to fibroblasts from immature rabbits. Furthermore, increased age was found to result in significant reductions in the baseline levels of collagen synthesis but not total protein synthesis. Additionally, collagen and total protein synthesis by MCL fibroblasts were significantly affected by the TGF-β1 dose, but not by the EGF dose. When fibroblasts were normalized to their own controls, the increases in collagen and total protein synthesis due to TGF-β1 and EGF for the senescent group were found to be greater than those for the skeletally immature rabbits at all doses. This demonstrates that MCL fibroblasts from senescent rabbits are responsive to growth factors.
AB - In this study, we examined the effects of age on collagen and total protein synthesis by ligament fibroblasts in response to growth factors. Three different doses of transforming growth factor-β1 (TGF-β1) or epidermal growth factor (EGF) were individually added to in vitro fibroblast cultures from the medial collateral ligament (MCL) of skeletally immature (age 3 months), mature (age 12 months) and senescent (age 48-51 months) rabbits. Analysis of the effects of age revealed that fibroblasts from senescent rabbits produced significantly less collagen in response to TGF-β1 or EGF stimulation when compared to fibroblasts from immature rabbits. Furthermore, increased age was found to result in significant reductions in the baseline levels of collagen synthesis but not total protein synthesis. Additionally, collagen and total protein synthesis by MCL fibroblasts were significantly affected by the TGF-β1 dose, but not by the EGF dose. When fibroblasts were normalized to their own controls, the increases in collagen and total protein synthesis due to TGF-β1 and EGF for the senescent group were found to be greater than those for the skeletally immature rabbits at all doses. This demonstrates that MCL fibroblasts from senescent rabbits are responsive to growth factors.
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U2 - 10.1016/S0047-6374(97)00049-3
DO - 10.1016/S0047-6374(97)00049-3
M3 - Article
C2 - 9226631
AN - SCOPUS:0030844344
SN - 0047-6374
VL - 97
SP - 121
EP - 130
JO - Mechanisms of Ageing and Development
JF - Mechanisms of Ageing and Development
IS - 2
ER -