The Effects of Histone H2B Ubiquitylations on the Nucleosome Structure and Internucleosomal Interactions

Bhaswati Sengupta, Mai Huynh, Charlotte B. Smith, Robert K. McGinty, Wladyslaw Krajewski, Tae Hee Lee

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Eukaryotic gene compaction takes place at multiple levels to package DNA to chromatin and chromosomes. Two of the most fundamental levels of DNA packaging are at the nucleosome and dinucleosome stacks. The nucleosome is the basic gene-packing unit and is composed of DNA wrapped around a histone core. Nucleosomes stack with one another for further compaction of DNA. The first stacking step leads to dinucleosome formation, which is driven by internucleosomal interactions between various parts of two nucleosomes. Histone proteins are rich targets for post-translational modifications, some of which affect the structure of the nucleosome and the interactions between nucleosomes. These effects are often implicated in the regulation of various genomic transactions. In particular, histone H2B ubiquitylation has been associated with facilitated transcription and hexasome formation. Here, we employed semi-synthetically ubiquitylated histone H2B and single-molecule FRET to investigate the effects of H2B ubiquitylations at lysine 34 (H2BK34) and lysine 120 (H2BK120) on the structure of the nucleosome and the interactions between two nucleosomes. Our results suggest that H2BK34 ubiquitylation widens the DNA gyre gap in the nucleosome and stabilizes long- and short-range internucleosomal interactions while H2BK120 ubiquitylation does not affect the nucleosome structure or internucleosomal interactions. These results suggest potential roles for H2B ubiquitylations in facilitated transcription and hexasome formation while maintaining the structural integrity of chromatin.

Original languageEnglish (US)
Pages (from-to)2198-2205
Number of pages8
JournalBiochemistry
Volume61
Issue number20
DOIs
StatePublished - Oct 18 2022

All Science Journal Classification (ASJC) codes

  • Biochemistry

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